The Skyline Team is excited to present this tutorial webinar series designed to help you get the most out of Skyline targeted proteomics software. Skyline Team members and guest researchers share their experience in these webinars and explain Mass Spectrometry topics through the lens of the Skyline software environment.

Each webinar has its own page (linked below) containing a recording of the webinar and all related information. The webinar recordings are about 90 minutes long with the last 30 minutes dedicated to Questions and Answers from the attending audience. Additionally, each webinar page contains the presentations, supporting tutorial data, and other related information. Even the most experienced Skyline users are likely to learn something new.

2025

New!
#25: Comparing Acquisition Methods

(Jan 2025)

2024

#24: Skyline for Lipidomics

(Nov 2024)

#23: Using Skyline Live Reports

(Sept 2024)

2023 & 2021

#22: Using DIA Data To Create SRM Methods

(Mar 2023)

#21: Analysis of diaPASEF Data

(Dec 2021)

#20: Using Skyline Batch for Large-Scale DIA

(July 2021)

2020 & 2018

#19: Ion Mobility Spectrum Filtering

(April 2020)

#18: DIA Data Analysis Revisited

(April 2020)

#17: PRM Method Dev and Data Analysis

(Jan 2018)

2017 & 2016

#16: Small Molecule Reseach

(Nov 2017)

#15: Optimizing Large Scale DIA

(Apr 2017)

#14: Large Scale DIA

(Jan 2017)

#13: Calibrated Quantification

(Apr 2016)

2015

#12: Isotope Labeled Standards

(Dec)

#11: Panorama and Panorama Public

(Oct)

#10: Working with Modifications

(Sep)

#9: PRM for PTM Studies

(Jul)

#8: DDA to Targeted: Differential Statistics

(Jun)

#7: iRT Retention Time Prediction

(May)

#6: Effective Data Processing

(Apr)

#5: Targeted Method Refinement

(Mar)

#4: Targeted Method Design

(Feb)

#3: PRM Targeted Proteomics Using Full-Scan MS

(Jan)

2014

#2: Jump Start DIA Analysis with DDA Data

(Dec)

#1: Getting the Most Out of DDA Data

(Nov)