Get hands-on experience setting up an acquisition method to quantify 31 peptides from 19 proteins of interest in murine fibroblasts using Parallel Reaction Monitoring (PRM). Work with three biological replicates measured in 9 runs on a Thermo Fusion mass spectrometer employing the Orbitrap mass analyzer. (44 pages)

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* - written on v24.1

Also, see our paper in Analytical Chemistry (please cite)
Multiplexed, Scheduled, High-Resolution Parallel Reaction Monitoring on a Full Scan QqTOF Instrument with Integrated Data-Dependent and Targeted Mass Spectrometric Workflows
[abstract]

On July 21, 2015, the Skyline Team produced Webinar #9: PRM for PTM Studies more advanced discussion that covered using PRM data from a study of modifications on histones.
[webinar 9]
On January 16, 2018, the Skyline Team produced Webinar #17: PRM Method Development and Data Analysis a more complete and modern PRM tutorial using Thermo Fusion data.
[webinar 17]