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Tutorials

Skyline

Tutorials

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Try one of these tutorials, and get hands-on experience using Skyline with real data.

Introductory

Targeted Method Editing (26 pages)
Targeted Method Refinement (28 pages)
Grouped Study Data Processing (70 pages)
Existing & Quantitative Experiments (43 pages)

 

Full-Scan Acquisition Data

MS1 Full-Scan Filtering (41 pages)
DDA Search for MS1 Filtering (19 pages)
Parallel Reaction Monitoring (PRM) (40 pages)
Basic Data Independent Acquisition (40 pages)
Analysis of DIA/SWATH Data (32 pages)
Analysis of diaPASEF Data (36 pages)

 

Small Molecules

Small Molecule Targets (10 pages)
Small Molecule Method Development (37 pages)
Small Mol. Multidimension Spec. Lib. (23 pages)
Small Molecule Quantification (27 pages)
Hi-Res Metabolomics (17 pages)

 

Advanced Topics

Absolute Quantification (19 pages)
Custom Reports (33 pages)
Advanced Peak Picking Models (28 pages)
iRT Retention Time Prediction (36 pages)
Collision Energy Optimization (12 pages)
Ion Mobility Spectrum Filtering (26 pages)
Spectral Library Explorer (22 pages)
Audit Logging (23 pages)

 

ETH Course Tutorials 2016 & 2018 (Skyline SRM/PRM/DIA + MSstats + mProphet tutorials with exercises)
SRM Course ETH Targeted Proteomics Course

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