Skyline Targeted Mass Spec Environment 
Targeted Proteomics CourseRegister now for the UW Targeted Proteomics Course
this summer in Seattle, WA
Skyline WebinarsWatch the video from the latest Skyline Tutorial Webinar
PRM Method Development and Data Analysis with Skyline

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Join Us

Spring Design and Analysis of Quantitative Proteomic Experiments at US HUPO in Minneapolis (March 10 - 11) - Register Now!

Spring Case Studies in Quantitative Proteomics Workshop at ASMS in San Diego (June 2 -3, 2018) - Description - Register Now!

Summer SWATH/DIA Course at ETH, Zurich. (July 2 - 6, 2018) Register now!

New! Summer Targeted Proteomics Course at University of Washington, Seattle WA. (July 30 - Aug. 3, 2018) - Register Now!

Save the Dates: Registration Coming

Spring Skyline User Group Meeting at ASMS in San Diego (June 3, 2018)

New! Fall Duke University, Durham, NC (September 17 - 21, 2018)

New! Fall Shanghai (October 22 - 23, 2018)

Fall Targeted Proteomics Course at PRBB, Barcelona (November 12-16, 2018)


Spring Targeted Proteomics Course at Buck Institute in the north San Francisco Bay area, CA. (April 2 - 6, 2018)

Spring Computation & Statistics for Mass Spec & Proteomics Course at Northeastern U. Boston, MA (April 30 - May 11, 2018)


Winter Targeted Proteomics Workshop at IIT Bombay, Mumbai, India (February 24 - 27, 2018)

Winter Pre-Lorne Workshop at University of Melbourne, Australia (January 29-31, 2018)

Winter Practical Proteomics with Skyline Short Course at MSACL, Palm Springs, CA (January 21-23, 2018)

More Events

Watch the Videos
Week-long Targeted Proteomics
Course 2016

Recently Published

Sharma, Mollecular & Cellular Proteomics - Panorama Public: A public repository for quantitative data sets processed in Skyline

Henderson, Clinical Chemistry - Skyline Performs as Well as Vendor Software in the Quantitative Analysis of Serum 25-Hydroxy Vitamin D and Vitamin D Binding Globulin

Pino, Mass Spectrometry Reviews - The Skyline ecosystem: Informatics for quantitative mass spectrometry proteomics

Bereman, Journal of Proteome Research - An Automated Pipeline to Monitor System Performance in Liquid Chromatography Tandem Mass Spectrometry Proteomic Experiments

Egertson, Nature Protocols - Multiplexed peptide analysis using data-independent acquisition and Skyline

More Publications


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Get Involved 

Make a tax-deductible contribution to the Skyline project this year through the University of Washington Foundation

See what the community is saying about Skyline - and add your statement!

Other ways you can help


Skyline adoption and use information

Create a new project for your lab or group on PanoramaWeb hosted by the University of Washington

Skyline source code is available under Apache 2.0 License and part of the ProteoWizard project (mzML and mzXML conversion)

Spectral Library Links