Skyline Targeted Mass Spec Environment 
Skyline User Group MeetingWatch the presentations from the Skyline User Group Meeting at ASMS
Indianapolis, IN - Sunday, June 4th, 2017
Skyline Webinars Watch the Video from the latest Skyline Tutorial Webinar
Optimizing Large Scale DIA with Skyline

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Winter Pre-Lorne Workshop at University of Melbourne, Australia (January 29-31, 2018) - Register Now!


Fall Targeted Proteomics Course at PRBB, Barcelona (November 13-17, 2017)


Fall Quantitative Proteomic Workshop at HUPO World Congress Dublin, Ireland (Sept 16-17, 2017)

Summer Targeted Proteomics Course at University of Washington Seattle WA (July 24-28, 2017)

Summer DIA/SWATH Proteomics Course at ETH, Zurich (June 26-30, 2017)

Summer Skyline User Group Meeting at ASMS, Indianapolis, IN (June 4, 2017) - watch the videos

Summer Short Course #21: Case Studies in Quantitative Proteomics at ASMS, Indianapolis, IN (June 3-4, 2017)

Spring Computation & Statistics for Targeted Proteomics Course at Northeastern University Boston, MA (May 1-3, 2017)

Spring Targeted Proteomics Course at Buck Institute in the north San Francisco Bay area, CA. (February 27th - March 3rd, 2017)

Spring Design and Analysis of Quantitative Proteomic Experiments at US HUPO San Diego, CA (March 18-19, 2017)

Fall 2016 ACGT Proteomics Symposium and Skyline Workshop at WITS Univeristy in Johanessburg, SA (December 5-8, 2016)

Fall Targeted Proteomics Course at PRBB, Barcelona (November 14-18, 2016)

Summer European Summer School on: Advanced Proteomics in Bressanone/Brixen, Italy (July 31 - August 6, 2016)

Summer Targeted Proteomics Course at University of Washington Seattle WA (July 18-22, 2016)

Summer Skyline User Group Meeting at ASMS, San Antonio, TX (June 5, 2016) - watch the videos

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Skyline User Group Meeting

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Week-long Targeted Proteomics
Course 2016

Recently Published

Pino, Mass Spectrometry Reviews - The Skyline ecosystem: Informatics for quantitative mass spectrometry proteomics

Bereman, Journal of Proteome Research - An Automated Pipeline to Monitor System Performance in Liquid Chromatography Tandem Mass Spectrometry Proteomic Experiments

Egertson, Nature Protocols - Multiplexed peptide analysis using data-independent acquisition and Skyline

Abbatiello, Mollecular Cellular Proteomics - Large-scale inter-laboratory study to develop, analytically validate and apply highly multiplexed, quantitative peptide assays to measure cancer-relevant proteins in plasma

Sharma, Journal of Proteome Research - Panorama: A Targeted Proteomics Knowledge Base

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Make a tax-deductible contribution to the Skyline project this year through the University of Washington Foundation

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Other ways you can help


Skyline adoption and use information

Create a new project for your lab or group on PanoramaWeb hosted by the University of Washington

Skyline source code is available under Apache 2.0 License and part of the ProteoWizard project (mzML and mzXML conversion)

Spectral Library Links