Skyline Targeted Mass Spec Environment 
Targeted Proteomics CourseRegister NOW for the May Institute for Computation & Statistics
for Mass Spectrometry & Proteomics
at the Northeastern University this spring.
Skyline WebinarsWatch the video from the latest Skyline Tutorial Webinar
PRM Method Development and Data Analysis with Skyline

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Join Us

Winter Practical Proteomics with Skyline Short Course at MSACL, Palm Springs, CA (January 21-23, 2018) - Register Now!

Winter Targeted Proteomics Workshop at IIT Bombay, Mumbai, India (February 24 - 27, 2018) - Register Now!

Spring Computation & Statistics for Mass Spec & Proteomics Course at Northeastern U. Boston, MA (April 30 - May 11, 2018) - Register Now!


Winter Pre-Lorne Workshop at University of Melbourne, Australia (January 29-31, 2018)

Spring Targeted Proteomics Course at Buck Institute in the north San Francisco Bay area, CA. (April 2 - 6, 2018)


Fall Targeted Proteomics Course at PRBB, Barcelona (November 13-17, 2017)

Fall Quantitative Proteomic Workshop at HUPO World Congress Dublin, Ireland (Sept 16-17, 2017)

Summer Targeted Proteomics Course at University of Washington Seattle WA (July 24-28, 2017)

Summer DIA/SWATH Proteomics Course at ETH, Zurich (June 26-30, 2017)

Summer Skyline User Group Meeting at ASMS, Indianapolis, IN (June 4, 2017) - watch the videos

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Watch the Videos
Skyline User Group Meeting

Watch the Videos
Week-long Targeted Proteomics
Course 2016

Recently Published

Henderson, Clinical Chemistry - Skyline Performs as Well as Vendor Software in the Quantitative Analysis of Serum 25-Hydroxy Vitamin D and Vitamin D Binding Globulin

Pino, Mass Spectrometry Reviews - The Skyline ecosystem: Informatics for quantitative mass spectrometry proteomics

Bereman, Journal of Proteome Research - An Automated Pipeline to Monitor System Performance in Liquid Chromatography Tandem Mass Spectrometry Proteomic Experiments

Egertson, Nature Protocols - Multiplexed peptide analysis using data-independent acquisition and Skyline

Sharma, Journal of Proteome Research - Panorama: A Targeted Proteomics Knowledge Base

More Publications


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Get Involved 

Make a tax-deductible contribution to the Skyline project this year through the University of Washington Foundation

See what the community is saying about Skyline - and add your statement!

Other ways you can help


Skyline adoption and use information

Create a new project for your lab or group on PanoramaWeb hosted by the University of Washington

Skyline source code is available under Apache 2.0 License and part of the ProteoWizard project (mzML and mzXML conversion)

Spectral Library Links