Get hands-on experience using Skyline with Excel to estimate the absolute molecular quantities of peptides in your experiments. (15 pages)
* - written on Skyline v1.1, updated for v1.4, updated for calibration features in v3.5
On April 15, 2016, the Skyline Team produced Webinar #13: Calibrated Quantification with Skyline, another great resource for this topic.
Also, see our paper in Nature Methods
Rapid empirical discovery of optimal peptides for targeted proteomics