Ion Mobility Spectrum Filtering


Get hands-on experience using Skyline to work IMS-TOF data. Train a drift-time predictor for BSA spiked into yeast, and see how ion mobility separation improves the selectivity of chromagram extraction in complex data. Learn how to work with ion mobility data in Skyline and explore the 3D (m/z, IMS, intensity) spectra produced by IMS-enabled mass spectrometers. (26 pages)


* - written on Skyline v3.7, updated for v20.2

On April 23rd, 2020, the Skyline Team produced Webinar #19: Ion Mobility Spectrum Filtering in Skyline, another great resource for this advanced topic.

Also, see our paper in Journal of The American Society for Mass Spectrometry (please cite)
Using Skyline to Analyze Data-Containing Liquid Chromatography, Ion Mobility Spectrometry, and Mass Spectrometry Dimensions

Also, see Brendan's presentation at the 2015 Agilent User Meeting at ASMS
Also, see Erin's presentation slides from the 2015 Skyline User Meeting at ASMS
Also, see the full data set on Panorama Public

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