Get hands-on experience working with data independent acquisition (DIA) data, using a workflow that utilizes DIA and DDA runs acquired on the same instrument in series. Define and export a DIA isolation scheme. Build a spectral library from DDA data acquired before the DIA runs for the experiment. Choose peptides and transitions for a target set of proteins based on the spectral library. Import and analyze related DIA runs in Skyline to master a simple workflow for beginning to work with DIA. (40 pages)


* - written on v2.6

On November 18th, 2014, the Skyline Team produced Webinar #2: Jump Start DIA Analysis with DDA Data in Skyline, another great resource for working with DIA data in Skyline.

On January 25, 2017 the Skyline Team produced Webinar #14: Large Scale DIA with Skyline, which highlighted the additional research and workflows developed in the 28 months since our first DIA webinar.





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