Perform a targeted analysis in Skyline with PRM, DIA and DDA measurement data. The four samples used for this tutorial were generated by mixing HeLa protein digest with PROCAL peptide standard at defined concentrations. Each sample was measured with three technical replicate injections in PRM, DIA and DDA mode on a Quadrupole-Orbitrap-Iontrap (Tribrid) mass spectrometer (Eclipse, Thermo). (38 pages)
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* - written on v25.1
On January 21, 2025, the Skyline Team produced Webinar #25: Comparing Acquisition Methods with Skyline covering this tutorial on Skyline 24.1.
[webinar 25]
