jobs

This jobs board allows employers to post open positions where experience with Skyline is desired.  Please read the following guidelines for using this service: 

  • All visitors can view open listings.
  • Please do not try to "reply" to the posting. Visit the link within the posting for information on how to apply for an open position. 
  • To receive email notifications about new postings: 
    • Click on the down triangle in the right-hand corner of the Listings section below 
    • Click Email > Preferences, select the All option under Send email notifications for these postings.  
    • Then select whether you would like to receive a notification with each new posting (Individual) which is recommended or a Daily Digest (summary).  
    • Click Update to save your preferences. 
  • See Job Posting Instructions for details on how to post to this jobs board.

We hope this job board helps to bring together employers and job seekers within the Skyline user community.

Showing: all postings
Assoc.Scientist - Proteomics/Immunopeptidomics
robert salzler 2019-03-18

Regeneron is looking for a highly motivated Associate Scientist who will leverage our state-of-the-art facilities and technology to perform qualitative and quantitative mass spectrometry experiments focusing on autoimmune, infectious disease and oncology targets. The successful candidate’s primary responsibility will be to utilize the latest mass spectrometry techniques for the discovery and validation of novel viral- and cancer-specific antigens from human tissues and cell lines as well as to develop innovative techniques and investigate new technologies to enhance our target discovery efforts.
The successful candidate will operate state-of-the-art mass spectrometry to analyze patient derived samples. Daily activities will include of tissue preparations, affinity purification, peptide purification, and the analysis of mass spectrometry data. Further responsibilities will be the integration of whole exome sequencing and RNA seq data with mass spectrometry results, developing proteomic databases for the target discovery efforts, and validation of results. Previous experience with mass spectrometry is a highly desirable prerequisite.

Job Duties:
• Participation in the day-to-day operation of Orbitrap Fusion Lumos mass spectrometers
• Engage in method development and optimization
• Preparation of MS samples from a variety of sample types including sample pre-fractionation and proteolytic digestion
• Affinity purification of endogenous peptides from a variety of different samples (i.e. patient tissue biopsies, animal specimens, and cell culture)
• Sample analysis and fractionation using HPLC techniques
• Work closely within and across scientific disciplines to design and perform analyses
• Present data packages and conclusions to internal and external collaborators

Requirements:
• PhD in biology, biotechnology or medicine.
• Basic knowledge of operating an HPLC and mass spectrometer (LC-MS)
• Knowledge in immunology and cancer research
• Experience in basic MS sample preparation and analysis
• Experience with immunoprecipitation sample preparation techniques
• Mass Spectrometric data analysis using Xcalibur, PEAKS, Skyline and general DB search engine tools
• Expertise in large-scale data analysis of NGS (Whole Exome sequencing, RNA-seq, and MS proteomics) is a plus.
• The position requires the ability to work in interdisciplinary projects, independently take responsibility of your project, strong teamwork and communication skills, reliability, attention to detail and effective time management.

Please apply using the link below
https://careers.regeneron.com/job/REGEA0026307889/Assoc-Scientist-Proteomics-Immunopeptidomics

view posting
Research Scientist-Proteomics
Zac 2019-03-08

Description
We are seeking an experienced and results oriented researcher to take on a role as Research Scientist. This is a hands-on position and a significant amount of bench time is expected.
Rapid Novor Inc is a Kitchener-Waterloo, Ontario based start up biotech company in Canada dedicated to developing unparalleled analytical technologies to significantly improve early stage drug research and development in the bio-pharmaceutical sector. Specializing in the field of mass spectrometry-based proteomics, the Novor team provides antibody protein sequencing solutions to researchers in pharmaceutical companies, contract research organizations and academic research institutions around the globe. We efficiently deliver accurate, comprehensive results, allowing researchers to rapidly make decisions that move their science forward.
Opportunities for Learning
Our office is located in the Waterloo Accelerator Centre. Our team members have the opportunities to work with an array of experts in Sales, Marketing, PR, etc, who will provide mentorship and coaching to help us succeed.
The core technology of Rapid Novor radiates from the purposefully built de novo protein sequencing program suite developed by the founders. The team also includes renowned experts in the field of mass spec proteomics. You will have the opportunities to discuss and learn directly from those experts to sharpen your technical skills.
Responsibilities
• Conduct research and method development aligned with the company goals
• Targeted quantitative proteomics - Develop and implement new and existing methods for highly sensitive quantitation in biofluids
• Sample preparation - optimize target enrichment strategies, refine enzyme digestion and develop new high throughput assay formats
• Quality control - ensure all developed assays are accurate, reproducible and robust
• Operate, maintain and troubleshoot mass spectrometers and ancillary peptide/protein separation equipment
• Maintain good laboratory records
• Data analysis, interpretation, and visualization of data
• Review relevant proteomics literature and remain current with technological advancements in the field
• Present data and findings to senior management
• Work both independently and collaboratively with other R&D members in a team setting

Requirements
• PhD in biochemistry, analytical chemistry or related field, or MSc with at least 2 years post-degree experience in a protein chemistry related field
• Good theoretical understanding of protein biochemistry and strong practical knowledge of sample preparation and separation chemistry for peptides and proteins
• Experiences in targeted proteomics (PRM, SRM and DIA) method development and data analysis is a plus
• Knowledge and experience in immunology or immune oncology is desirable but not essential
• Strong written and oral communication skills
• Ability to conduct and manage projects with minimal supervision
• Ability to collaborate with other team members

Do you possess these qualities? If so we want to hear from you!
To apply
Please send a cover letter and resume to cirwin@rapidnovor.com

view posting
Postdoc in proteomics and chromatin biology
Simone Sidoli 2019-03-05

Two postdoctoral positions are available to join the Sidoli lab, a new proteomics lab at the Albert Einstein College of Medicine (New York, NY, USA). The lab is finalizing its renovation; it will be equipped with state-of-the-art mass spectrometry and currently the only Bio Array Matrix system in the United States to grow organoids. Find out more at our website: www.sidolilab.org

We are looking for candidates with training in mass spectrometry, proteomics, cell biology or epigenetics. You will be joining a very collaborative environment, highly focused in training the next generation of mass spectrometrists.

Our projects are focused on developing new quantitative strategies to study chromatin dynamics, complex assembly on chromatin and new epigenetics targets for therapy. We use a variety of techniques, mostly adopting non-conventional approaches in mass spectrometry. We are also investing in automation and throughput to move the analysis of proteomes into translational science.

If you are interested, please send your CV including names and contact information for three references, and a short description of your training and future research goals to Simone Sidoli (simone.sidoli@gmail.com).

view posting
Senior Scientist, Targeted Proteomics, DENALI
gastarita 2019-02-21

Denali Therapeutics is dedicated to developing breakthrough therapies for neurodegenerative diseases through the application of a deep understanding of degeneration biology and principles of translational medicine.

We are seeking a scientist or senior scientist with expertise in quantitative proteomics to contribute to our expanding drug discovery efforts.

Responsibilities:

Develop, validate and implement targeted proteomics assays using liquid chromatography-mass spectrometry to support therapeutic and biomarker discovery
Perform data acquisition and processing, interpret data and communicate results to collaborators
Develop, maintain, and manage SOPs for sample preparation and targeted proteomics
Manage external proteomics collaborations with academic collaborators and contract research organizations
Work closely with cross-functional projects teams in a fast-paced, highly interactive environment

Requirements:

PhD in biology, protein chemistry, proteomics, mass spectrometry, (bio)analytical chemistry or a related discipline

5 years of postgraduate experience using mass spectrometry for protein analysis
Familiarity with untargeted proteomics and analysis of post-translational modifications
Hands-on experience with quantitative LC/MS and triple quadrupole instruments
Significant experience using Skyline software for targeted method development and quantification
Strong publication records demonstrating expertise in the quantification of proteins in biological samples
Collaborative, highly organized, detail-oriented

If interested please contact Giuseppe Astarita at gastarita@gmail.com or apply online at https://www.denalitherapeutics.com/job?id=1544401

view posting
Biogen - Scientist in Biomarker assay development
omar mabrouk 2019-02-15

This is a position that is not yet posted, but I wanted to see if anyone in the Skyline community is particularly good at protein quant using Orbitraps with an emphasis on IP-LC-MS. Assay qualification skills are very important here and we use Skyline routinely when doing analysis. Below is a draft of the job description. You can contact me at omar.mabrouk@biogen.com if you want to learn more

Scientist I/II- Discovery and Early Development Biomarkers
*Job Description
The Discovery and Early Development Biomarkers group, part of Biogen’s Research and Early Development department is seeking a highly motivated scientist to join a dynamic team engaged in the discovery and progression of novel biomarkers for neurodegenerative diseases. Responsibilities include executing mass spectrometry-based experiments to identify, evaluate, and characterize relevant fluid biomarkers in both preclinical and clinical samples. The successful candidate will be capable of multitasking and effective planning to consistently meet deadlines. Effective communication and collaboration are crucial.
*Qualifications

  1. Preference will be given to candidates with hands on experience in developing high resolution (Orbitrap) LC-MS assays, including immunoprecipitation LC-MS for the detection of proteins in biological samples.
  2. Familiarity with Skyline, Thermo Xcalibur, Proteome Discoverer, AB Sciex Analyst.
  3. Excellent assay troubleshooting skills.
  4. Familiarity with neuroscience, including knowledge of neurodegenerative diseases (AD, PD, ALS) is considered a plus but not required
  5. Demonstrated track record of providing support activities for multiple research projects.
  6. Solid work ethic and demonstrated record of productivity such as participating in peer reviewed publications or scientific poster presentations.
    *Responsibilities:
    • Protein biomarker assay development using state of the art liquid-chromatography-mass spectrometry platforms including nano LC, orbitrap (Q-Exactive, Q-Exactive +, Q-Exactive HF/HFX, Fusion) and UPLC-QQQ MS (AB Sciex)
     Sample preparation (tissues and cell lysates) for protein quantification
     Biotinylation and various bead-antibody conjugation techniques
     Identification and selection of antibodies immunoprecipitation prior to LC-MS analysis
     Identification of novel techniques and strategies to enhance assay sensitivity
    • Recognize and troubleshoot data anomalies to maintain high data quality.
    • Timely recording keeping and data entry into Electronic Lab Notebook.
    • Prepare, review, and deliver scientific presentations for internal/external use.
    • Interact with various project teams as a liaison to share and distribute information.
    • Commitment to help the team achieve deliverables and meet aggressive timelines in a fast-paced environment.

*Education
PhD with 3+ years of experience

view posting
Senior Proteomics Scientist
DWANG 2019-01-15

Newomics Inc., a venture-backed and NIH-funded commercial-stage biotechnology company in the San Francisco Bay Area, develops and commercializes enabling platforms and solutions for precision medicine. Newomics has unique infrastructure and resources to make major contributions to personalized healthcare. For more information, visit www.newomics.com.

Newomics has an immediate opening for a full-time Senior Proteomics Scientist position. Outstanding candidates will be considered at the Principal Scientist level. Newomics will provide a comprehensive compensation package including salary, equity, and benefits such as PTO, medical/dental/vision and 401(k) plan etc.

Responsibilities
• Lead the development and commercialization of cutting-edge multiomics assays
• Support the development and commercialization of Newomics microfluidic chips
• Develop global multiomics workflows for assays of biological samples
• Perform LC-MS analysis of proteins, peptides, and small molecules in biological samples
• Work with team members on LC-MS applications of microfluidic devices
• Perform data analysis with statistical and bioinformatics tools
• Participate in the general lab and instrument maintenance
• Document all experimental records and file reports
• Publish papers in peer-reviewed journals

Qualifications
• A PhD degree in mass spectrometry, analytical chemistry or a related field
• 5+ years of experience in industry or academia after obtaining a PhD degree
• A strong publication record in proteomics or multiomics
• Well-versed in Thermo Scientific mass spectrometers such as QE series and QQQ
• Demonstrated experience in developing global proteomics assays for biological samples
• Extensive hands-on experience in biological sample preparation for nanoflow LC-MS
• In-depth understanding of both hardware and software of nanoflow LC-electrospray MS
• Proficiency in LC-MS related software and data analysis
• Experience in both top-down and bottom-up proteomics is a strong plus
• Experience in working with microfluidic devices is a strong plus
• Highly motivated, a team player, a quick learner, and a problem solver
• Excellent oral and written communication skills
• Ability to work in a fast-paced startup environment
• Eligibility to work in the United States

To apply
Please send a cover letter, resume with the publication list, and contact information for three references, to jobs@newomics.com

view posting