The Proteomics Resource Center (PRC) at The Rockefeller University masters many aspects of liquid chromatography/mass spectrometry applied to metabolomics and proteomics. Examples include de novo sequencing, and quantitative analysis based on label-free quantitation as well as heavy isotope labeled samples. The PRC is equipped with Orbitrap-type mass spectrometers (high resolution/high mass accuracy) and can separate analytes using both nano and high-flow liquid chromatography. The Center is a source for help with planning of mass spectrometry-based experiments, and it has the capability to offer in-depth collaborative analysis. Also, the PRC is operated in a boutique style, encouraging the scientists of the Center to work closely with users to create a tailored approach to fit the many unique questions that can be answered by analytical mass spectrometry. The Center is equipped with multiple high-performance servers for data analysis.

Overview
The Proteomics Resource Center is looking for a Senior Research Support Specialist to lead PRC staff for metabolomics services. Metabolomics platform services include, but are not limited to, polar metabolite and lipid profiling (targeted and untargeted), absolute quantification of small molecules and flux analysis. Under very limited supervision, the Senior Research Support Specialist will demonstrate and deliver expertise in metabolomics tools and strategies. The Senior Research Support Specialist will provide excellent guidance in experimental design and data interpretation for metabolomics, and informational and technical support to center users, including assistance in experimental design and analysis.

Responsibilities
Responsibilities include:
• Maintain, operate and manage LC-MS and related equipment
• Interact directly with and advise investigators on technical questions related to metabolomics
• Develop, improve and implement metabolomics technologies and methods
• Process, analyze, and interpret metabolomics data and report findings to the Director and investigators
• Maintain and document standard operating procedures; revise as needed.
• Track and maintain relevant Center documentation, including metabolomics service billing and requisitioning information
• Monitor and maintain laboratory appearance
• Mentor and train junior PRC staff members in metabolomics techniques and analysis
• Day to day supervision of junior PRC staff members’ performance of metabolomics related activities
• Participate in relevant meetings/conferences

Qualifications
Required:
• Ph.D. in metabolomics or other related field with at least two years relevant laboratory experience
• Proven competency with liquid chromatography and mass spectrometry
• Experience and competency in relevant instrumentation and supporting software
• Demonstrate ability to stay current with relevant techniques and technologies
• Strong communication, organizational, and interpersonal skills

Preferred:
• Skyline and LipidSearch analysis software experience
• Experience with Orbitrap IQ-X and Q-Exactive Plus Mass Spectrometers
• Experience with Vanquish LC system
• HILIC experience

Rockefeller University does not discriminate in employment on the basis of race, color, religion, sex (including pregnancy, gender identity), national origin, political affiliation, sexual orientation, marital status, disability, genetic information, age, membership in an employee organization, retaliation, parental status, military service or other non-merit factor. All qualified applicants will receive consideration for employment without regard to the characteristics listed above.

Compensation Range: USD $90,000.00/Yr. to USD $120,000.00/Yr.

Apply via: https://careers-rockefelleruniversity.icims.com/jobs/1244/senior-research-support-specialist-|-proteomics-resource-center/job?mode=view&mobile=false&width=785&height=500&bga=true&needsRedirect=false&jan1offset=-300&jun1offset=-240

Commencing in 2024, a position is available at the Department of Biochemistry within the Faculty of Medicine, offering the possibility to establish an independent research group. The position is initially limited to 5 years, but it can be made permanent upon fulfilling the corresponding requirements. The junior group leader will conduct own research projects and develop new applications in protein mass spectrometry. In addition the scientist will provide technical mass spectrometry support for other researchers. The position will be supported by a technician. Active participation in teaching, as well as acquiring third-party funding, is expected. The opportunity for habilitation is provided upon fulfilling the necessary requirements. The salary is in accordance with the collective labour agreement of the State of Hessen.

For more information, please contact Prof. Lienhard Schmitz

https://www.uni-giessen.de/de/fbz/fb11/institute/biochemie/forschung/corefacilityproteomics/indexcorefacilityproteomics

The Research & Development Division Global BioAnalytical (BA) group at Merck within the Preclinical Development department, (PCD), is seeking students for a 6 month Co-op experience. The Global BA group at our company provides quantitative analysis of biologically derived samples for all our company's discovery and development programs.

Learning opportunities for the prospective Co-op are:

• Involvement in the development and execution of protein/peptide quantitative proteomic assays by LC-MS/MS for therapeutic biologic products.
• Gaining hands-on experience in using automation platforms, mass spectrometers, UPLC or UHPLC, immunoprecipitation (IP), solid phase extraction (SPE).
• Hands-on exposure to laboratory work in the form of bioanalysis experimentation utilizing different platforms.

In addition to laboratory skills, the Co-ops will have the opportunity to be engaged in scientific discussion and interpretation of data. The Co-op student will have an opportunity to present their research results in both oral and written formats.

We are seeking Co-op candidates at all levels with strong academic performance, communication skills, teamwork, and the ability to work in a cross-collaborative environment.

Location: West Point, PA

This is a full-time Co-op position. Housing subsidy is not available as part of this program and must be funded 100% by the student.

Required Experience and Skills:

• Candidates must be currently enrolled undergraduate senior student pursuing a bachelor’s degree OR a currently enrolled graduate student pursuing a master’s or PhD Chemistry, Biochemistry, Pharmaceutical Sciences, Pharmacology, Pharmacokinetics, Biology, Immunology, Molecular Biology, Pharmaceutical Sciences, or related field
• Candidates must be available to work full-time for up to (24) consecutive weeks beginning in May or June of 2023
• Candidates must have completed at least (2) years of studies toward bachelor’s degree by June 2023

Additional Experience and Skills:

• Candidates should have basic laboratory skills to carry out biochemical techniques for sample preparation, such as, basic pipetting, dilution techniques, ligand binding assays (ELISA, etc.)
• Candidates should have hands on experience in sample preparation and biological techniques such as protein, peptide, preparation for mass spectrometry analysis as well as cell culture, gel electrophoresis, protein digestion and western blotting. LC MS protein analysis or proteomics experience is a plus
• Candidates should have basic knowledge of general chemistry, organic chemistry, and protein chemistry
• Candidates should have basic understanding of pharmacokinetics and immunogenicity
• Candidates should have the ability to follow procedures and thoroughly document experiments
• Candidates should be highly motivated, self-starter and significant interest in scientific research
• Candidates should have strong interpersonal, communication, problem solving, and collaboration skills
• Candidates should have strong time management to allow parallel pursuit of multiple objectives

Please apply at this link -
https://jobs.merck.com/us/en/job/R227014/2023-Global-Bioanalytical-Co-op

or send me an email (with resume/CV) at shivangi.awasthi@merck.com to learn more.

The Huttenhain Lab in the Department of Molecular & Cellular Physiology is looking for postdoctoral fellows to study mechanisms of intracellular signal integration through G protein-coupled receptors (GPCRs) using cutting edge mass spectrometry-based proteomics, functional genomics, and molecular biology techniques. Applicants with expertise and interest in either (1) mass spectrometry-based proteomics, (2) molecular and cell biology including neuroscience, GPCRs, functional genomics or related techniques, or (3) computational biology are encouraged to apply.

Lab overview
The communication between cells and their environment depends on decoding of extracellular cues into intracellular signaling cascades that are integrated through dynamic and context-specific networks driving the cellular responses. Given the complexity and dynamic state of signaling networks, the current understanding of their constituents and how they are spatiotemporally regulated in the cell as a result of a specific input is incomplete.
The Huttenhain lab, which will launch in April 2023 in the Molecular & Cellular Physiology Department (https://med.stanford.edu/mcp.html) at Stanford Medicine, studies mechanisms of intracellular signal integration through G protein-coupled receptors (GPCRs), the largest family of membrane receptors mediating most of our physiological responses to hormones, neurotransmitters and environmental stimulants. We employ an interdisciplinary approach to probe, model, and predict how signaling network dynamics translate extracellular cues sensed by GPCRs into specific phenotypic outputs. Developing quantitative proteomics approaches to capture the spatiotemporal organization of signaling networks and combining these with functional genomics and molecular biology techniques to study their impact on physiology, we aim to better understand GPCR signaling and to provide a solid foundation for the design and testing of novel therapeutics targeting GPCRs with higher specificity and efficacy.

Relevant publications
Lobingier B, Hüttenhain R, Eichel K, Ting AY, Miller KB, von Zastrow M, Krogan NJ. (2017) An approach to spatiotemporally resolve protein interaction networks in living cells. Cell 169, 350-360. PMC5616215.
Polacco BJ, Lobingier BT, Blythe EE, Abreu N, Xu J, Li Q, Naing ZZC, Shoichet BK, Levitz J, Krogan NJ, Von Zastrow M, Hüttenhain R. (2022) Profiling the diversity of agonist-selective effects on the proximal proteome environment of G protein-coupled receptors. bioRxiv 2022.03.28.486115

Specific postdoctoral projects may include
Develop quantitative proteomics approaches to map spatiotemporally resolved interaction and signaling networks of GPCRs.
Mapping signaling network dynamics of GPCRs in neuronal systems to uncover novel regulators and mechanisms of neuromodulation.
Discover mechanisms how diverse ligands targeting GPCRs elicit diverse phenotypic responses.
Develop computational approaches to model spatiotemporally resolved signaling networks.

Academic environment
Stanford University provides a highly stimulating scientific environment that fosters collaborations and the exchange of ideas within and across disciplines. The Molecular & Cellular Physiology Department (https://med.stanford.edu/mcp.html) offers a world-class scientific environment and supports postdoctoral scholars throughout their postdoc with mentoring and training programs, seminar series, and annual retreats. The Huttenhain lab is committed to foster an inclusive, respectful, and collaborative environment with passion for science and mentorship. Trainees will have access to an outstanding infrastructure and resources including dedicated state-of-the-art mass spectrometers.

Required Qualifications:
Ph.D. in life sciences, biomedical sciences, computer science, or a related discipline.
Experience and publication record in neuroscience, proteomics, genetics, molecular biology, and/or computational biology.
Ability to work well in a multidisciplinary, diverse, and collaborative research environment.
A genuine passion for science, and creative thinking.
Being self-motivated.
Strong oral and written communication skills.
Required Application Materials:
Cover letter that includes a description of relevant research experience and research interests.
Curriculum vitae (including a complete publication list).
Names and contact information for 2-3 references.

Post-Doctoral Research Associate - Food Allergen Proteomics
https://employment.unl.edu/postings/84330

The Department of Food Science and Technology at the University of Nebraska-Lincoln (UNL) Institute of Agriculture and Natural Resources (IANR) is seeking applications for a highly motivated and enthusiastic post-doctoral research associate. This 12-month (calendar year), postdoc researcher appointment will plan, conduct, and supervise research on food allergen proteomics. In particular, the research will be focused on conducting protein mass spectrometry experiments to identify, detect, quantify, and characterize food allergens. The apportionment is 100% research. This position will be located in Lincoln, Nebraska.

The individual will be expected to conduct research both independently and as part of a team. Duties will also include collaborative supervision and management of multiple, shared high-resolution mass spectrometers. Contribution to the supervision of graduate and undergraduate students is also anticipated.

Preparation and publication of manuscripts in peer-reviewed journals or other appropriate outlets is expected. The individual may also have the opportunity to make presentations at regional, national, or international scientific meetings. In addition, the individual may be asked to contribute to the preparation of grant proposals and progress reports related to the research projects.

Recognizing that diversity enhances creativity, innovation, impact, and a sense of belonging, the Institute of Agriculture and Natural Resources (IANR) and the Department of Food Science and Technology are committed to creating learning, research, Extension programming, and work environments that are inclusive of all forms of diversity. Consistent with the University’s N2025 Strategic Plan, every person and every interaction are treated as important to our collective well-being and our ability to deliver on our mission.

Required Qualifications
• Ph.D. in Food Science, Biochemistry, Biology, Chemistry, Bioinformatics, Plant Science, or a related field.

Preferred Qualifications
• Experience with proteomics and protein mass spectrometry, including targeted proteomics method development.
• Experience with protein purification techniques.
• Familiarity with informatics tools for proteomics (for example, Mascot, Skyline, Progenesis, and instrument vendor software).

Review of applications will begin April 17, 2023 and will continue until the position is filled or the search is closed. To view details of the position and create an application, go to https://employment.unl.edu, requisition F_230023. Click “Apply for this Job” and complete the information form. Attach the following documents:

1. A letter of interest that describes your qualifications for the job and anticipated contributions.
2. Your curriculum vitae.
3. Contact information for three professional references.

As an EO/AA employer, the University of Nebraska considers qualified applicants for employment without regard to race, color, ethnicity, national origin, sex, pregnancy, sexual orientation, gender identity, religion, disability, age, genetic information, veteran status, marital status, and/or political affiliation. See https://www.unl.edu/equity/notice-nondiscrimination.