Skyline Targeted Mass Spec Environment 
Targeted Proteomics CourseRegister NOW for Practical Proteomics with Skyline Short Course
at MSACL, Palm Springs, CA this January.
Skyline WebinarsWatch the Video from the latest Skyline Tutorial Webinar
Small Molecule Research With Skyline

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Join Us

Winter Practical Proteomics with Skyline Short Course at MSACL, Palm Springs, CA (January 21-23, 2018) - Register Now!

Spring Targeted Proteomics Course at Buck Institute in the north San Francisco Bay area, CA. (April 2 - 6, 2018) - Register Now!

Spring Computation & Statistics for Mass Spec & Proteomics Course at Northeastern U. Boston, MA (April 30 - May 11, 2018) - Register Now!


Winter Pre-Lorne Workshop at University of Melbourne, Australia (January 29-31, 2018)


Fall Targeted Proteomics Course at PRBB, Barcelona (November 13-17, 2017)

Fall Quantitative Proteomic Workshop at HUPO World Congress Dublin, Ireland (Sept 16-17, 2017)

Summer Targeted Proteomics Course at University of Washington Seattle WA (July 24-28, 2017)

Summer DIA/SWATH Proteomics Course at ETH, Zurich (June 26-30, 2017)

Summer Skyline User Group Meeting at ASMS, Indianapolis, IN (June 4, 2017) - watch the videos

Summer Short Course #21: Case Studies in Quantitative Proteomics at ASMS, Indianapolis, IN (June 3-4, 2017)

More Events

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Skyline User Group Meeting

Watch the Videos
Week-long Targeted Proteomics
Course 2016

Recently Published

Pino, Mass Spectrometry Reviews - The Skyline ecosystem: Informatics for quantitative mass spectrometry proteomics

Bereman, Journal of Proteome Research - An Automated Pipeline to Monitor System Performance in Liquid Chromatography Tandem Mass Spectrometry Proteomic Experiments

Egertson, Nature Protocols - Multiplexed peptide analysis using data-independent acquisition and Skyline

Abbatiello, Mollecular Cellular Proteomics - Large-scale inter-laboratory study to develop, analytically validate and apply highly multiplexed, quantitative peptide assays to measure cancer-relevant proteins in plasma

Sharma, Journal of Proteome Research - Panorama: A Targeted Proteomics Knowledge Base

More Publications


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Make a tax-deductible contribution to the Skyline project this year through the University of Washington Foundation

See what the community is saying about Skyline - and add your statement!

Other ways you can help


Skyline adoption and use information

Create a new project for your lab or group on PanoramaWeb hosted by the University of Washington

Skyline source code is available under Apache 2.0 License and part of the ProteoWizard project (mzML and mzXML conversion)

Spectral Library Links