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Allow integration of multiple isotopes for high molecular weight fragments (Top-Down PRM)
v delcourt
2018-10-10 03:48
Nick Shulman
2025-05-16 20:15
Parent-ion scanning
r bagshaw
2015-07-22 09:38
dhass
2025-05-16 10:13
autoqc cannot authorize
wphipps5
2025-05-15 17:35
Brian Connolly
2025-05-16 07:36
peak picking
heyang
2025-05-12 15:02
Nick Shulman
2025-05-15 17:03
Normalization Using Heavy Internal Standard
weitmanm
2025-05-06 10:43
weitmanm
2025-05-15 11:25
Retain only human proteins
akhilabrai
2025-05-14 02:52
akhilabrai
2025-05-15 03:19
Guidance Required on Exporting mzIdentML File from Skyline
cvadival
2025-05-09 07:51
cvadival
2025-05-13 06:08
ddMS2 (LC-QExactive, Orbitrap by Thermo)
yohan seol
2025-05-09 07:09
Nick Shulman
2025-05-12 08:33
GC-MS SIM: Molecules and Fragments not found
stefanie orter
2025-02-18 05:54
kdharmon0511
2025-05-09 22:26
Oxonium ion profiles
mmakrid
2025-04-29 11:19
Juan C. Rojas E.
2025-05-09 04:06
PRM-Quantification
akhilabrai
2025-05-07 08:06
akhilabrai
2025-05-08 02:49
Integration threshold
a rocher
2025-04-01 23:02
Nick Shulman
2025-05-07 07:01
IQ-X mzML files cannot be read
Yasin
2025-05-04 05:33
Matt Chambers
2025-05-06 12:24
Request for Assistance with Chromatogram Export Functionalities
1150767964
2025-04-09 01:27
SebastianM
2025-05-06 09:10
not enough transition ions
creitzel
2025-04-30 07:16
creitzel
2025-05-06 06:38
timsTOF Bruker error Maximum expected frame size exceeded.
a l hipgrave ederveen
2025-02-24 07:50
a l hipgrave ederveen
2025-05-05 23:38
skyline can not read the LC information of .lcd file as MRM for peptide
fengleink
2025-05-05 22:19
fengleink
2025-05-05 22:19
Issue with utilizing MSInspector
edoud
2023-08-25 12:28
edoud
2025-05-05 11:53
Standards with different concentrations for analytes
sheher mohsin
2025-04-30 16:19
sheher mohsin
2025-05-03 16:27
Guidance on Exporting mzTab or mzIdentML from Skyline for PRIDE Submission
cvadival
2025-05-02 08:43
cvadival
2025-05-02 08:43
Creating spectral library using dia-PASEF data from timsTOF HT
rmokoena1
2025-05-02 07:53
rmokoena1
2025-05-02 07:53
Bruker diaPASEF .d import failure: Failed importing results file [...]'16495.d'. error reading spectrum merged=15875 frame=5716 scanStart=1 scanEnd=702
Lindsay Pino
2025-04-16 10:01
Matt Chambers
2025-05-01 08:14
Peak Integration: PRM
owagne2
2025-04-29 13:42
owagne2
2025-04-30 09:26
Q-value in groupwise comparisons
nreimers
2025-04-24 17:10
Nick Shulman
2025-04-29 19:30
Adding Precursor [M-1] trace to all precursors by default
sean jensen
2024-04-25 21:39
Nick Shulman
2025-04-24 20:19
Agilent LCMS 6495D methods using a template method specifying large molecule iFunnel mode are exported with standard mode specified.
mmarx
2025-04-24 09:44
Rita Chupalov
2025-04-24 13:16
How to import .resultsbfp into Skyline to create a peptide library
shivangi awasthi15418
2025-04-24 09:12
Nick Shulman
2025-04-24 09:54
Uploading timstof files to create skyline library
susmitaghosh191996
2025-04-17 03:43
Nick Shulman
2025-04-23 07:45
Connecting file name information to list annotation
edoud
2025-04-22 17:54
edoud
2025-04-23 06:44
import of parquet spectral library from DIANN 2.0 and above into skyline
dkueltz
2025-04-15 22:52
dkueltz
2025-04-17 10:15
timsTOF PASEF PRM Import Issues
robwsprung
2025-04-15 08:18
robwsprung
2025-04-15 12:15
Quantitative Proteomics with Skyline course
anhdao darcy
2025-04-11 13:43
Mike MacCoss
2025-04-13 11:25
EncyclopeDIA error
sscott13
2025-04-11 09:54
Nick Shulman
2025-04-11 11:17
Normalization Methods
jaedon sadler
2025-04-11 08:33
jaedon sadler
2025-04-11 08:33
Avant Garde R Package Installation Failure
sgoulding
2023-10-05 14:05
lincolnh
2025-04-10 14:30
DIA-NN .speclib support
Tobi
2020-05-27 03:03
Daniel Fochtman
2025-04-09 03:07
Global peptide, can't see the options described in the previous answer
yurubi
2025-04-08 13:07
yurubi
2025-04-08 15:14
About AlphaPeptDeep predicted spectral library
ma ziliang from tp
2025-04-07 22:49
ma ziliang from tp
2025-04-07 22:49
95 % Confidence interval for LOD
Zac
2025-04-07 07:24
Mike MacCoss
2025-04-07 08:13
Inserting 15N-labeled products into Skyline
chemistry Jedi
2025-04-07 00:58
Brian Pratt
2025-04-07 02:14
No digestion
PP
2025-04-04 10:42
Nick Shulman
2025-04-04 10:55
Quantification for small molecules by using C of IS without cal. curve
ismailovadh
2025-03-18 02:50
Nick Shulman
2025-04-04 07:42
DIA setup problem
gor bazoyan
2025-03-03 16:46
gor bazoyan
2025-04-03 10:34
pasting peptide sequences associated with a particular protein
pavel shliaha
2025-04-03 04:35
pavel shliaha
2025-04-03 08:55
export all chromatograms visualisation from skyline
ismailovadh
2025-04-03 07:06
Nick Shulman
2025-04-03 07:18
skyline openning dia-nn data seems slow
eva borras
2025-04-03 02:51
eva borras
2025-04-03 02:51
problems with NIST library msp
ismailovadh
2025-03-31 04:20
ismailovadh
2025-04-03 01:49
opening.d files on skyline
NBL
2025-03-24 04:29
Nick Shulman
2025-04-02 16:56
Data Normalization with Heavy Peptide along with Global Standard
Dhaval Patel
2025-03-31 15:12
Nick Shulman
2025-03-31 20:04
No chromatograms when importing Spectronaut file into Skyline
tang yun
2025-03-31 18:33
Nick Shulman
2025-03-31 19:30
QQQ with transition list - Chromaogram information unavailable
jihyunk
2025-03-31 13:19
Nick Shulman
2025-03-31 14:41
DDA with triple quadrupole
gioele_vi
2025-03-14 05:40
gioele_vi
2025-03-31 01:49
Error importing GNPS spectrum library
gioele_vi
2025-03-14 07:35
gioele_vi
2025-03-31 01:43
Error importing data (Agilent 6410B): Data Not found - deviceNameOrdinalNumber
gioele_vi
2025-02-25 08:03
gioele_vi
2025-03-31 01:41
Exporting scheduled MRM method to *.exp Masslynx
per larsson
2024-02-01 09:05
per larsson
2025-03-29 15:53
Error during import files
wlgus00261
2025-03-28 10:30
wlgus00261
2025-03-28 13:05
GC MS Agilent import
daria makeeva
2024-11-25 07:48
Philipp Ternes
2025-03-28 02:54
IBAQ protein standards
mvm35
2025-03-26 09:04
Nick Shulman
2025-03-26 09:30
Skyline for small molecules : MS/MS visualisation
oceane delos
2025-03-25 08:28
Nick Shulman
2025-03-26 08:02
Skyline does not recognize peptide peak
erbutler
2025-03-25 08:32
Nick Shulman
2025-03-25 15:11
error with re-opening skyline files
bella bruszel
2025-03-25 05:01
Nick Shulman
2025-03-25 10:53
Avant-garde - a sommelier for label-free PRM?
tete0723
2025-03-25 07:27
Brian Pratt
2025-03-25 09:29
Segemented MRM - Batch Script
anirudhkashyap511
2025-03-24 08:59
Nick Shulman
2025-03-24 09:21
Edit > Manage Results > Rename
lwolfe_colostate
2016-10-12 11:54
Nick Shulman
2025-03-23 21:18
Is there a way to import search results from compound discoverer?
licky lck
2025-03-21 05:05
licky lck
2025-03-23 04:26
Cal curves at different concentrations
g blanco26202
2025-03-21 13:47
Nick Shulman
2025-03-21 14:03
Peptide quantification with single point internal calibration
ahat erpan
2025-03-21 08:59
Nick Shulman
2025-03-21 10:11
Import speclib from DIANN with customized non-unimod modifications
gianin thomann
2024-04-23 04:20
Matt Chambers
2025-03-21 08:24
Can you import a UV trace too?
baileyls
2025-03-20 06:40
Nick Shulman
2025-03-20 15:08
List of instruments not visible in Instrument Tab under Transition Settings
vipinj1985
2025-03-19 05:38
Mike MacCoss
2025-03-19 13:18
Skyline-Ardia Link
jaedon sadler
2025-03-18 14:05
Nick Shulman
2025-03-18 14:19
dot p value from which MS2 spectra
heyang
2025-03-18 13:11
Nick Shulman
2025-03-18 14:16
No matching mod for PTM when using MaxQuant
jonas elsborg
2025-03-13 09:59
jonas elsborg
2025-03-18 04:50
Quant with internal standard for small molecules
sheher mohsin
2025-03-17 10:56
sheher mohsin
2025-03-18 00:05
accuracy of light and heavy precursors differing by 1 Da only
rameshkr
2025-02-28 04:02
Nick Shulman
2025-03-17 21:51
Peak Area Ratio to Heavy Calculation
erints
2025-03-14 13:58
erints
2025-03-17 11:42
noise level of a peak
heyang
2025-03-13 09:40
heyang
2025-03-17 07:34
Saving Skyline files
Joerg
2025-03-17 01:48
Nick Shulman
2025-03-17 04:55
Target small molecule
a das
2025-03-16 00:48
Nick Shulman
2025-03-16 18:18
Custom Modifications using SkylineRunner/CMD?
dominickyeo
2025-03-14 19:10
Nick Shulman
2025-03-15 10:54
PRM conductor Importing error "Index was outside the bounds of the array"
704743362
2025-03-15 06:13
Nick Shulman
2025-03-15 10:31
Error by file opening
alessandra tozzi1
2025-03-14 04:10
Nick Shulman
2025-03-14 05:20
No target proteins after FASTA import
lincolnh
2025-03-13 08:42
lincolnh
2025-03-13 12:24
Error: All of the external standards are missing one or more peaks
szczepankaluzny
2025-03-12 09:03
szczepankaluzny
2025-03-13 08:40
Retention Times > Molecule Comparison Graph: Show one Molecule List only
helmi
2025-03-12 07:29
helmi
2025-03-12 08:14
Chromatogram information unavailable
Pa Nic 14
2024-02-23 13:02
Nick Shulman
2025-03-11 11:29
FAIMS data processing in Skyline
sofani7
2025-03-10 15:05
Brian Pratt
2025-03-11 11:25
Understanding lines and shade in skyline file
heyang
2025-03-10 12:22
heyang
2025-03-11 05:47
Small Molecule Analysis and Calibration Curve
weitmanm
2025-03-04 12:55
weitmanm
2025-03-10 17:28
Reporting MS1 monoisotopic peak areas in document/report
sweaver4
2025-03-10 09:11
Nick Shulman
2025-03-10 09:31
Chromatogram Information Unavailable
sjmzy23
2025-03-08 06:27
sjmzy23
2025-03-08 10:52
Inquiry on Bottom-Up Analysis Using Skyline for Protein/Peptide Identification
wei ng2
2025-03-06 18:18
Nick Shulman
2025-03-06 18:55
Quantification query!
VM26
2025-03-04 21:54
VM26
2025-03-05 21:44
PFAS EPA Method 1633 Quantification Using Internal Standards
hknguyen
2025-03-05 10:38
hknguyen
2025-03-05 10:38
DDA search
NBL
2025-02-20 08:17
Nick Shulman
2025-03-05 07:57
Agilent (GC) MS (Single Quad) data import / processing
j d bleeker
2025-03-04 05:41
j d bleeker
2025-03-05 06:01
rdotp score implementation
TY
2025-03-03 23:47
Nick Shulman
2025-03-04 21:12
Polarity mismatch error during import of polarity-switching raw file
p ivanov
2025-03-01 00:44
Brian Pratt
2025-03-03 12:52
Skyline-daily unplugged v24.1.1.398
Juan C. Rojas E.
2025-03-02 05:26
Juan C. Rojas E.
2025-03-03 02:20
background height
rameshkr
2025-03-01 12:21
Mike MacCoss
2025-03-01 19:14