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Inquiry on Bottom-Up Analysis Using Skyline for Protein/Peptide Identificationwei ng22025-03-06 18:18wei ng22025-07-03 00:18
eDR Full Scan Datawes rogers2025-07-02 13:10wes rogers2025-07-02 13:10
Peptide Import DDA library from Fragpipe Spectrum id format and file idnaomi osullivan331272025-07-02 06:30naomi osullivan331272025-07-02 06:30
ID retention times incorrectjdemeter2025-06-19 19:56Nick Shulman2025-06-30 20:19
Ion Match Tolerance MS1 Data Waters Xevo QTOFtateh2025-06-30 11:08Nick Shulman2025-06-30 12:28
Exporting Skyline Method to UNIFI for Waters Xevo QTof G3philipp peslalz2025-06-30 02:09philipp peslalz2025-06-30 02:09
Live Report Terminology QueryDhaval Patel2025-06-27 14:10Nick Shulman2025-06-27 14:16
Prm conductor / expert reviewer erroringjoel steele280282025-06-24 20:55philip remes2025-06-27 12:00
signal to noise ratio/ noise informationmansour bounouba2025-06-27 01:48Mike MacCoss2025-06-27 08:47
Agilent GCMS Transition Settingsbri bibel2025-06-24 08:06bri bibel2025-06-26 12:40
Mass error ppm in report and RSD duplicatechemistry Jedi2025-06-26 01:05chemistry Jedi2025-06-26 01:05
Early-run m/z + IM calibration (timsTOF / any Q-TOF ?)v delcourt2022-06-30 07:16Brian Pratt2025-06-25 09:56
2025 UW Course (July)hscprotein2025-06-23 22:40Mark Belanger2025-06-24 10:45
import MS1 data failed using Skyline (64-bit) 25.1.0.142 (7401c644b4)zhijun cao2025-06-23 11:37zhijun cao2025-06-24 07:26
Chromatogram Information Unavailableolivia schwartz2025-06-23 16:10Nick Shulman2025-06-23 16:40
No automatic pick children for isotopic envelopekaramj334052025-06-23 11:17Nick Shulman2025-06-23 12:13
Unable to connect Skyline to UNIFIbkraft2025-06-19 08:59bkraft2025-06-23 08:11
Having Issues Importing Demultiplexed data into Skyline after update to v. 25.1nikkib52025-06-20 11:56Nick Shulman2025-06-20 23:50
Problem creating library using PD3.1 results.davidl@ohsu.edu2025-06-06 11:23Nick Shulman2025-06-20 12:08
Reimporting the same files into previously analyzed Skyline file (From the last year) resulted in different areasanokhina2025-06-09 14:04Brendan MacLean2025-06-20 11:12
DIANN 2.0 speclib import failurematthew.r.russell@manchester.ac.uk2025-06-17 06:25Matt Chambers2025-06-20 11:11
method export for "Thermo Stellar"qin fu2025-06-06 15:11Brendan MacLean2025-06-20 11:05
Long molecule name in plots, only first letters shownper larsson2025-06-18 05:21per larsson2025-06-18 05:21
GPF Method generation for Stellarmaithreyan kuppusamy2025-06-06 00:30maithreyan kuppusamy2025-06-17 11:06
Extracting adducts in Skyline with Proteomics interfaceJosh Baeza2025-06-16 11:56Brian Pratt2025-06-17 08:19
Advanced peak picking model issue: the scoring is not correct for internal standard mixed samplelxiiaanog2025-06-16 00:20lxiiaanog2025-06-17 00:00
Can skyr files define Number Format?TY2025-06-16 18:54Nick Shulman2025-06-16 19:24
no isolation list export possible for ZenoToFFchi nguyen2025-06-12 10:19Nick Shulman2025-06-12 20:51
peak pickingheyang2025-05-12 15:02Nick Shulman2025-06-12 12:12
Direct Injectionsam lord2025-06-10 09:37sam lord2025-06-12 07:08
Can Skyline calculate CCS error between Explicit and IM Library CCS values?Stelios Papazoglou2025-06-12 03:09Stelios Papazoglou2025-06-12 03:09
opening mzml file for small moleculesrobyn eisert2025-06-10 10:53Nick Shulman2025-06-10 15:02
Importing .wiff data files into Skyline (molecule interface)adeline shanker2025-06-10 10:26adeline shanker2025-06-10 10:26
Import DIA Peptide Search results from Spectronautn prust2023-03-29 06:12Nick Shulman2025-06-10 09:10
Standard curve regression line not showingstefan broeer2025-06-05 21:11stefan broeer2025-06-10 00:06
Reimporting the same files into previously analyzed Skyline file (From the last year) resulted in different areas.anokhina2025-06-09 14:01anokhina2025-06-09 14:01
Calibration Curve LLOD and LLOQsam lord2025-06-04 01:46sam lord2025-06-05 08:48
[M+5] isotope peaks for small C-13 labelled moleculesCM2025-06-04 04:10CM2025-06-04 08:46
SCIEX QTRAP MRM+EPIRoberto2025-06-04 07:43Roberto2025-06-04 07:43
How to set skyline to analyze LTQ SIM dataguo xue2025-05-29 03:12guo xue2025-05-30 01:11
Masses for charge state 1 are not appearing.PP2025-05-22 12:31PP2025-05-28 09:30
CE optimized spectral libraries not based on intensities but transition rank distributionmax gilbert2025-05-28 02:40max gilbert2025-05-28 02:40
Mark many transitions as non-quantitativelincolnh2025-05-20 11:03lincolnh2025-05-27 15:02
error loading fragpipe results into skylinejdemeter2025-05-27 12:01Nick Shulman2025-05-27 14:10
Generate Calibration Curve from specific samples (standard curve samples)helmi2025-05-27 03:22Nick Shulman2025-05-27 07:38
Performing quantitation of native analyte concentration WRT labeled standard in a document gridkapil mandrah2025-05-26 07:40kapil mandrah2025-05-27 06:01
Integration thresholda rocher2025-04-01 23:02a rocher2025-05-26 23:50
Feature request: Logistic fit function for quantificationMT2025-05-23 02:45MT2025-05-26 01:00
import of parquet spectral library from DIANN 2.0 and above into skylinedkueltz2025-04-15 22:52Todd2025-05-23 14:11
v25.1 support on Sciex dataheyang2025-05-23 07:08heyang2025-05-23 07:08
Offline (Unplugged) AutoQC Installerarthur nuccio2025-05-21 12:13Vagisha Sharma2025-05-22 10:15
changing name/location of raw datatannous2017-11-08 11:40skyfall2025-05-21 07:46
Parent-ion scanningr bagshaw2015-07-22 09:38Brian Pratt2025-05-20 08:56
in silico spectral librarymazzouny2025-05-19 14:27Nick Shulman2025-05-19 15:15
Allow integration of multiple isotopes for high molecular weight fragments (Top-Down PRM)v delcourt2018-10-10 03:48Nick Shulman2025-05-16 20:15
autoqc cannot authorizewphipps52025-05-15 17:35Brian Connolly2025-05-16 07:36
Normalization Using Heavy Internal Standardweitmanm2025-05-06 10:43weitmanm2025-05-15 11:25
Retain only human proteinsakhilabrai2025-05-14 02:52akhilabrai2025-05-15 03:19
Guidance Required on Exporting mzIdentML File from Skylinecvadival2025-05-09 07:51cvadival2025-05-13 06:08
ddMS2 (LC-QExactive, Orbitrap by Thermo)yohan seol2025-05-09 07:09Nick Shulman2025-05-12 08:33
GC-MS SIM: Molecules and Fragments not foundstefanie orter2025-02-18 05:54kdharmon05112025-05-09 22:26
Oxonium ion profilesmmakrid2025-04-29 11:19Juan C. Rojas E.2025-05-09 04:06
PRM-Quantificationakhilabrai2025-05-07 08:06akhilabrai2025-05-08 02:49
IQ-X mzML files cannot be readYasin2025-05-04 05:33Matt Chambers2025-05-06 12:24
Request for Assistance with Chromatogram Export Functionalities11507679642025-04-09 01:27SebastianM2025-05-06 09:10
not enough transition ionscreitzel2025-04-30 07:16creitzel2025-05-06 06:38
timsTOF Bruker error Maximum expected frame size exceeded.a l hipgrave ederveen2025-02-24 07:50a l hipgrave ederveen2025-05-05 23:38
skyline can not read the LC information of .lcd file as MRM for peptidefengleink2025-05-05 22:19fengleink2025-05-05 22:19
Issue with utilizing MSInspectoredoud2023-08-25 12:28edoud2025-05-05 11:53
Standards with different concentrations for analytessheher mohsin2025-04-30 16:19sheher mohsin2025-05-03 16:27
Guidance on Exporting mzTab or mzIdentML from Skyline for PRIDE Submissioncvadival2025-05-02 08:43cvadival2025-05-02 08:43
Creating spectral library using dia-PASEF data from timsTOF HTrmokoena12025-05-02 07:53rmokoena12025-05-02 07:53
Bruker diaPASEF .d import failure: Failed importing results file [...]'16495.d'. error reading spectrum merged=15875 frame=5716 scanStart=1 scanEnd=702Lindsay Pino2025-04-16 10:01Matt Chambers2025-05-01 08:14
Peak Integration: PRMowagne22025-04-29 13:42owagne22025-04-30 09:26
Q-value in groupwise comparisonsnreimers2025-04-24 17:10Nick Shulman2025-04-29 19:30
Adding Precursor [M-1] trace to all precursors by defaultsean jensen2024-04-25 21:39Nick Shulman2025-04-24 20:19
Agilent LCMS 6495D methods using a template method specifying large molecule iFunnel mode are exported with standard mode specified.mmarx2025-04-24 09:44Rita Chupalov2025-04-24 13:16
How to import .resultsbfp into Skyline to create a peptide libraryshivangi awasthi154182025-04-24 09:12Nick Shulman2025-04-24 09:54
Uploading timstof files to create skyline librarysusmitaghosh1919962025-04-17 03:43Nick Shulman2025-04-23 07:45
Connecting file name information to list annotationedoud2025-04-22 17:54edoud2025-04-23 06:44
timsTOF PASEF PRM Import Issuesrobwsprung2025-04-15 08:18robwsprung2025-04-15 12:15
Quantitative Proteomics with Skyline courseanhdao darcy2025-04-11 13:43Mike MacCoss2025-04-13 11:25
EncyclopeDIA errorsscott132025-04-11 09:54Nick Shulman2025-04-11 11:17
Normalization Methodsjaedon sadler2025-04-11 08:33jaedon sadler2025-04-11 08:33
Avant Garde R Package Installation Failuresgoulding2023-10-05 14:05lincolnh2025-04-10 14:30
DIA-NN .speclib supportTobi2020-05-27 03:03Daniel Fochtman2025-04-09 03:07
Global peptide, can't see the options described in the previous answeryurubi2025-04-08 13:07yurubi2025-04-08 15:14
About AlphaPeptDeep predicted spectral libraryma ziliang from tp2025-04-07 22:49ma ziliang from tp2025-04-07 22:49
95 % Confidence interval for LODZac2025-04-07 07:24Mike MacCoss2025-04-07 08:13
Inserting 15N-labeled products into Skylinechemistry Jedi2025-04-07 00:58Brian Pratt2025-04-07 02:14
No digestionPP2025-04-04 10:42Nick Shulman2025-04-04 10:55
Quantification for small molecules by using C of IS without cal. curveismailovadh2025-03-18 02:50Nick Shulman2025-04-04 07:42
DIA setup problemgor bazoyan2025-03-03 16:46gor bazoyan2025-04-03 10:34
pasting peptide sequences associated with a particular proteinpavel shliaha2025-04-03 04:35pavel shliaha2025-04-03 08:55
export all chromatograms visualisation from skylineismailovadh2025-04-03 07:06Nick Shulman2025-04-03 07:18
skyline openning dia-nn data seems sloweva borras2025-04-03 02:51eva borras2025-04-03 02:51
problems with NIST library mspismailovadh2025-03-31 04:20ismailovadh2025-04-03 01:49
opening.d files on skylineNBL2025-03-24 04:29Nick Shulman2025-04-02 16:56
Data Normalization with Heavy Peptide along with Global StandardDhaval Patel2025-03-31 15:12Nick Shulman2025-03-31 20:04
No chromatograms when importing Spectronaut file into Skylinetang yun2025-03-31 18:33Nick Shulman2025-03-31 19:30