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Strange peak boundaries using small molecule DDAJoerg2026-03-06 04:07Joerg2026-03-06 13:01
Skyline MSI optimization of mutlicharged ionsvalerie steiner2026-03-06 04:28Nick Shulman2026-03-06 07:13
Errors with loading Wiff files in profile modeJuan C. Rojas E.2026-03-03 07:56Nick Shulman2026-03-05 07:28
Importing MRM data from Sciex ZenoTOF 8600Jiny2026-02-10 07:59Matt Chambers2026-03-04 13:11
Question about plugins and Feature request: Logistic curve fitMT2026-03-04 02:05Brian Pratt2026-03-04 10:30
Blank samples used to build the calibration curveMathieuG_KI2026-03-03 07:01MathieuG_KI2026-03-03 07:58
Can't get a Calibration Curve - Error: All of the external standards missing one or more peak”markus kellmann2026-02-27 05:18markus kellmann2026-03-01 22:39
Precursor isotopes for negative ions appearing as M+1 and M+2Susan Abbatiello2026-02-26 09:24Brian Pratt2026-02-26 09:34
Skyline Technical Detailscjthorley2026-02-26 09:19cjthorley2026-02-26 09:19
Cannot import negative mode small molecule data from Waters instrumentsgaunitz2026-02-24 02:13sgaunitz2026-02-26 02:19
Thermo Stellar Adaptive RT with Skyline Issuesasilverberg2026-02-25 07:01Nick Shulman2026-02-25 09:58
CCS measured vs. CCS explicit (or library)tim causon2021-04-09 03:04Brian Pratt2026-02-24 09:46
Issues with Feature Detection from RAW PRM files without searching data firstnitheshperumal2025-12-04 10:29nitheshperumal2026-02-18 16:36
Skyline not seeing my Waters dataibrahim abouseada2026-02-17 09:48Nick Shulman2026-02-17 12:20
cannot read chromatogrammsmarie-claude menet242222026-02-13 07:39Nick Shulman2026-02-13 09:35
lipid creator/shimadzu 8050rliyana2026-02-11 10:18Brian Pratt2026-02-11 10:43
A low number of fragments are extracted, even if a high minimum product ion number is set.chiyy2026-02-09 16:20chiyy2026-02-10 15:58
Specifying Integration Boundariesjrenders2020-11-09 08:53apat2026-02-10 11:32
Issue displaying chromatogramseli2026-02-08 03:20eli2026-02-08 19:20
Inconsistent length between m/z and intensity array after extraction from RefSpectraPeaks table from .blib filechiyy2026-02-05 13:48chiyy2026-02-05 14:30
"Copy Data" on ChromatogramSChen2026-02-05 07:57SChen2026-02-05 09:51
Export the library retention time for matched peptides in document gridchiyy2026-02-04 17:18chiyy2026-02-04 19:59
I would like to change account emailmlane2026-02-02 06:40monica lane2026-02-02 13:47
Skyline freezing with glyco-N-DIA outputsarah shapley2026-01-29 09:47Nick Shulman2026-01-29 15:17
Bruker qTOF .damyzheng2026-01-28 15:33Brian Pratt (Claude)2026-01-29 13:14
Peak integration for all transitionsSChen2026-01-26 10:01Juan C. Rojas E.2026-01-29 10:32
Cannot replicate group comparison statistical test in Rkittinun l2026-01-28 18:42kittinun l2026-01-29 06:09
Peptide quantitationanhdao darcy2026-01-27 13:35Nick Shulman2026-01-27 15:10
Visualising PRM data, knowing the targets of interest, but without an experimental spectral librarychloe baldreki2026-01-27 08:21chloe baldreki2026-01-27 08:55
What is the minimum computer required for running this program?theetat2026-01-26 21:37Nick Shulman2026-01-27 05:33
Unchecked "Quantitative" option for the fragment ion but included the area values in an export fileleehyoungjoo2026-01-26 13:25Nick Shulman2026-01-26 13:59
transitions/GB RAM?warham2026-01-19 11:08warham2026-01-23 08:10
Incorrect quantification of low-intensity MRM peaks close to background noiseioanna kyritsi2026-01-20 01:00Nick Shulman2026-01-20 05:44
Ratio to Heavy is not providing values on the calibration curvemarharyta vasnietsova2026-01-19 01:32Nick Shulman2026-01-19 07:41
Skyline doesn't import data after DIA-NN ChromCacheBuildExceptionshubham2026-01-16 12:41shubham2026-01-18 15:19
is there a tutorial for TurnoverRclarkjnelson672026-01-16 19:11Nick Shulman2026-01-16 20:33
AutoQC config file locationphains@cmri.org.au2026-01-13 19:59phains@cmri.org.au2026-01-14 12:08
Truncated peakshadi pourhadi2026-01-12 09:08Nick Shulman2026-01-14 11:35
How to use "Protein priority file" from PRM conductor?Zimeng2026-01-14 02:54philip remes2026-01-14 07:50
Why my Skyline stopped working?zhaoty2025-12-17 18:20Nick Shulman2026-01-14 07:20
Problem extracting pressure information in Panorama for SSTDavid P2024-06-28 07:58Matt Chambers2026-01-13 13:16
MS data not supported1963102026-01-12 16:51Nick Shulman2026-01-12 17:07
Post-analysis internal calibration timsTOF data not loaded correctly in Skylinea l hipgrave ederveen2025-12-17 05:29Brian Pratt2026-01-06 09:17
Hardklor/bullseye parametersrj8@uw.edu2025-12-31 11:08rj8@uw.edu2026-01-01 15:00
Immunopeptidomics analysis on Skyline with DDA Bruker data after MHCquant2rohit 39772025-12-01 12:29Nick Shulman2025-12-23 17:02
Executing Skyline GUI with a .sky file already loadedsdelacam2025-12-18 02:17Nick Shulman2025-12-21 22:35
Importing DIA-NN into skylinerobyn eisert2025-12-18 12:26robyn eisert2025-12-18 13:03
Surrogate Calibration Curve Slopehelmi2025-12-16 01:26Nick Shulman2025-12-16 07:44
Bug in export with Explicit RT to waters .expper larsson2025-12-12 05:04per larsson2025-12-15 00:15
Peak boundary imputation on large datasetsTomas Vaisar2025-12-11 09:36Tomas Vaisar2025-12-11 13:15
skyline-daily not adding libraries to document via library explorerpoisson k2025-12-10 06:35Brian Pratt2025-12-10 13:25
Shimadzu 8060RX lcd contains no usable dataWesley Vermaelen2024-10-22 22:39Brian Pratt2025-12-08 17:34
w-ionseric bonneil2025-12-08 05:11Nick Shulman2025-12-08 08:42
timsTOF PRM cant see MS2 in skylineguo xue2025-12-02 20:50Nick Shulman2025-12-07 20:41
DIANN 2.2 results (from HPC) upload into Skyline (windows)Tomas Vaisar2025-12-05 15:19Tomas Vaisar2025-12-05 15:48
Cannot install skyline on work computer. Supportchris brown-22025-12-05 09:01chris brown-22025-12-05 09:47
URLDownloadToCacheFile error when installing Skyline and Skyline-Dailyaaron-maurais2025-12-03 10:26Nick Shulman2025-12-03 11:01
Visualizing Multiple Collision Energy MS/MS Profiles in Skyline from a Single tMS2 Filedilip singh402882025-12-02 12:51dilip singh402882025-12-03 06:29
Is a standard curve required for relative quantificationsam lord2025-11-28 02:50Mike MacCoss2025-11-28 20:08
PRM for phosphoproteomics34583903362025-11-27 01:35Nick Shulman2025-11-27 07:15
Cannot Open Files of Raw Data?sriazisekowski2025-11-26 12:09Brian Pratt2025-11-26 12:40
Strategy for establishing a PRM method for intact glycopeptides using Skyline?shulian132025-11-06 07:31shulian132025-11-26 00:57
Feature request: Peak Imputation WindowJuan C. Rojas E.2025-11-25 02:27Juan C. Rojas E.2025-11-25 02:27
idotp value for determing LODp de blaauw2025-11-25 02:00p de blaauw2025-11-25 02:00
Exporting Scheduled method using integrated RT?per larsson2025-11-24 09:19per larsson2025-11-24 10:37
Issue with CC Extrapolation Using Heavy Peptides Onlyangela peralbo2025-11-24 06:17Nick Shulman2025-11-24 07:34
Peptides not present in Skyline document after importing FragPipe resultstanja ziesmann2025-11-20 01:18tanja ziesmann2025-11-21 07:43
Small molecule isotopic peakSChen2025-11-12 10:54SChen2025-11-20 06:43
Expert Review - Thermodiogobor2025-11-19 06:54philip remes2025-11-19 09:55
Isolation offset PRM Astraljohannes fuchs133812025-11-19 02:41Nick Shulman2025-11-19 09:37
Export matched RTs and intensitieslincolnh2025-11-07 10:15Nick Shulman2025-11-18 15:51
Can I change the display format for LOQ numbers?TY2025-11-16 21:18TY2025-11-17 20:56
import a peptide list with RTlesurant2025-11-17 06:07Brian Pratt2025-11-17 11:09
Flat line instead of peak MRM transition for small moleculesyasmin kadyrbekova2025-11-14 03:14Nick Shulman2025-11-14 05:29
Error on line XX at column YYnikolaos psychogios2025-11-12 10:49Nick Shulman2025-11-12 13:24
Light to heavy ratio Calibration Curveps5622025-11-11 07:46ps5622025-11-12 13:06
Filter for N-glycanssarah shapley2025-11-11 13:18Juan C. Rojas E.2025-11-12 01:57
Transitions for cyclic dipeptidesge78faz2025-11-11 06:05ge78faz2025-11-12 00:53
Issue with importing Waters SONAR data to Skylinemanohar82025-10-29 23:34Brian Pratt2025-11-06 16:35
peptide containing cystineanhdao darcy2025-11-06 08:10Mike MacCoss2025-11-06 10:53
Mixed sample with 18 plex TMT for PRMSONGSONG2025-11-05 11:36Nick Shulman2025-11-06 02:25
show peak areas for proteins by prec/fragmentsjdemeter2025-11-05 19:33Nick Shulman2025-11-06 02:14
Calibration Curve for different analytessophia_foehr2023-06-07 06:06Michi2025-11-05 07:21
Multiple peaks viewa majcher2025-11-04 02:34Juan C. Rojas E.2025-11-05 02:16
Skyline to calculate LOD/LOQ from PRM datafloriana2025-11-03 03:43Nick Shulman2025-11-03 06:33
Feature request: Make the output from "Copy Data" from the Full Scan viewer more R/Python friendlymauraisa2025-10-28 13:38Brian Pratt2025-10-29 13:29
Reinclude already excluded transition.HongSang Choi2025-10-28 13:30HongSang Choi2025-10-29 07:20
Feature request: defining peptide modification isomersJuan C. Rojas E.2025-10-28 07:26Nick Shulman2025-10-28 16:15
Possibility to unlink the integration between light and heavycarolin huber2025-10-28 05:40Nick Shulman2025-10-28 16:03
ion mobility information missing in result exporthaiyan2025-10-27 06:35Nick Shulman2025-10-28 15:14
Skyline Small Molecules - Agilent NLS data import failingmariacristina petrella2025-10-08 07:34mariacristina petrella2025-10-28 12:40
import of parquet spectral library from DIANN 2.0 and above into skylinedkueltz2025-04-15 22:52andrea graziadei2025-10-27 06:37
Spectral library generation - is it neededmvm352025-10-21 06:03Mike MacCoss2025-10-23 08:58
No Collision Energy as deafult for isolation list export for Bruker timsTOF (+ request for a bonus feature)Daniel Fochtman2025-10-21 07:56Daniel Fochtman2025-10-21 07:56
Skyline Small Molecules - Relative Abundace for library transitionsguadalupe espadas2025-10-20 06:24guadalupe espadas2025-10-21 01:01
Importing DIANN libraryanirudhkashyap5112025-08-29 10:10Nick Shulman2025-10-20 16:44
Error uploading PRM .raw filemsalie416582025-10-20 09:31msalie416582025-10-20 12:39
Using average mass for intact proteinssoren2025-10-16 11:14Nick Shulman2025-10-16 11:23
Automation for peak picking for high sample numberorsolya bilkei2025-10-15 23:39Nick Shulman2025-10-16 06:38
Custom Collision Energy Equations defaulting to precursor m/z instead of interceptChris Ashwood2025-10-10 07:51Chris Ashwood2025-10-12 17:12