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Setting working dir
niklas40639
2025-09-12 08:39
Nick Shulman
2025-09-12 12:41
Transitions in PRM PASEF
susmitaghosh191996
2025-09-11 04:55
Nick Shulman
2025-09-11 11:38
Transitions in PRM PASEF
susmitaghosh191996
2025-09-11 04:45
susmitaghosh191996
2025-09-11 04:45
Pick integration with relative retention time
junyiqian
2025-09-10 19:52
junyiqian
2025-09-10 23:17
Unable to install Skyline
hudda khan63
2025-09-09 20:07
Nick Shulman
2025-09-09 21:00
安装时显示磁盘空间不足,应该需要多大的空间?怎么处理?
656053951
2025-09-08 19:17
656053951
2025-09-08 19:17
Surrogate standard area display
zahall
2025-09-05 08:16
Nick Shulman
2025-09-06 14:04
Can employee in R&D of a commercial company use Skyline?
george wang cal
2019-09-03 15:27
jacob36728
2025-09-05 07:43
Chromatogram Unavailable for TAG Features in SkylineCmd vs GUI
Harrison
2025-09-01 02:15
Harrison
2025-09-02 23:38
importing target and decoy libraries for crosslinked peptides
anzuki
2025-08-14 08:56
anzuki
2025-09-02 06:59
tSIM IQ-X wavy Chromatogram
gerrit hermann
2025-08-25 04:53
gerrit hermann
2025-09-01 01:02
DIA NN Paquet lib issue
haiyan
2025-08-30 13:38
haiyan
2025-08-30 13:38
Importing DIANN library
anirudhkashyap511
2025-08-29 10:10
Nick Shulman
2025-08-29 11:30
Import transition list from MRM assay
c frampas
2025-08-27 04:06
Nick Shulman
2025-08-29 07:00
Creating Library from Stellar GPF-DIA Fragpipe analysis Fails
maithreyan kuppusamy
2025-08-28 04:57
Nick Shulman
2025-08-28 16:13
Altis and method exporting
qin fu
2025-08-27 10:37
Nick Shulman
2025-08-27 15:07
Peptide Import DDA library from Fragpipe Spectrum id format and file id
naomi osullivan33127
2025-07-02 06:30
Todd
2025-08-27 11:51
PDResult import error
andrew williamson
2025-08-15 05:47
andrew williamson
2025-08-26 23:47
Unable to view the chromatogram on the results imported
v veerappan
2025-08-25 21:57
Nick Shulman
2025-08-25 22:45
为什么我安装不上这个软件啊
625276408
2025-08-23 00:41
Nick Shulman
2025-08-23 08:01
Trying to export method into MassLynx Xevo TQ-Absolute method with error
Susan Abbatiello
2025-08-21 18:49
Nick Shulman
2025-08-21 19:12
Issue with Skyline v25.1.0.142 Handling MS1 Data from DDA Files
Luisa Nieto Ramirez
2025-08-21 09:28
Nick Shulman
2025-08-21 10:09
skyline25.1.0.142 does not work in scn982
ryuta kobayashi
2025-08-20 19:14
ryuta kobayashi
2025-08-20 22:35
Video presentations from ASMS
karl fraser
2025-08-17 21:32
Brian Connolly
2025-08-20 08:47
Targeting M+2 for large peptides
paul grimsrud
2025-08-19 12:29
paul grimsrud
2025-08-19 13:32
GPF Method generation for Stellar
maithreyan kuppusamy
2025-06-06 00:30
philip remes
2025-08-14 23:33
small molecule transition list error
dspiciarich
2018-04-25 15:31
Nick Shulman
2025-08-14 17:01
Unable to get peak areas at respective CE duering CE optimization of SCFAs
ujjalkumar das
2025-08-13 13:33
ujjalkumar das
2025-08-14 13:24
**Unable to view small molecules analysed in ESI negative mode**
ejg29
2025-08-14 07:39
Brian Pratt
2025-08-14 09:53
Carafe library generation
Alejandro Brenes
2025-08-11 13:46
BoW
2025-08-13 09:07
Processing files for histone PTM analysis using .d files
lorenz donndorf
2025-08-13 02:39
Nick Shulman
2025-08-13 08:43
Define Regression Fit by Analyte in Document Grid
crystalpace519
2025-08-12 14:37
Nick Shulman
2025-08-12 18:31
apply peak boundaries to all replicates
simon daled
2025-08-12 05:18
simon daled
2025-08-12 07:55
Using Skyline for Hybrid-DIA files
andreas kienzle44094
2025-08-12 06:10
Nick Shulman
2025-08-12 07:51
Skyline identifying wrong peak in prm data
gkline
2025-08-11 15:56
Nick Shulman
2025-08-11 16:05
.mzid file not getting recognized
bhatti rushik
2025-08-11 03:09
Nick Shulman
2025-08-11 09:12
Isomers-
jm007@uw.edu
2025-08-06 13:22
Nick Shulman
2025-08-08 10:29
Chromatogram view stuck at Intensity of 10^9
katherine.wright@pfizer.com
2025-08-07 13:56
katherine.wright@pfizer.com
2025-08-08 07:39
small molecule quantitation with individually different compound concentrations
w h c huibers
2025-08-08 06:23
Nick Shulman
2025-08-08 07:13
Bug reports regarding the handling of large amounts of replicates
simow02
2025-07-22 06:11
simow02
2025-08-08 06:27
Shimadzu 8060RX lcd contains no usable data
Wesley Vermaelen
2024-10-22 22:39
Matt Chambers
2025-08-07 09:22
signal-to-noise (S/N) calculations
weitmanm
2025-08-06 07:31
Nick Shulman
2025-08-06 13:46
eDR Full Scan Data
wes rogers
2025-07-02 13:10
wes rogers
2025-08-06 07:25
Import peptide results with .msf and .pdresult files fails- Skyline-daily (64-bit) 25.1.1.147
Antrix Jain
2025-08-04 08:44
Antrix Jain
2025-08-05 11:56
Product ions chromatogram for peptide are unavailable
bhatt511
2025-08-05 08:57
Nick Shulman
2025-08-05 10:59
Cannot open Skyline file that I used today.
wulfmeyer vera
2025-07-31 02:37
Nick Shulman
2025-07-31 22:51
Small molecule PRM transition/molecule settings
jweinb9
2025-07-17 10:30
jweinb9
2025-07-30 19:14
MRM HR wiff file import parameter
ssy8827
2025-07-29 22:07
Nick Shulman
2025-07-29 22:41
Problems with intensity extractions
alejandro gomez toledo
2025-07-29 01:44
Nick Shulman
2025-07-29 05:46
Importing error "Index was outside the bounds of the array"
rschoenh@fhcrc.org
2017-08-29 07:25
Nick Shulman
2025-07-29 05:29
Background correction no longer displayed (absent?)
niklas40639
2025-07-24 15:22
niklas40639
2025-07-28 09:16
Precision in reports when using the command line
Will Thompson
2025-07-25 12:38
Will Thompson
2025-07-28 08:16
CE optimisation for PRM on TIMSTOF
robert.rparker
2025-07-28 04:11
robert.rparker
2025-07-28 04:11
GC MS Agilent import
daria makeeva
2024-11-25 07:48
Philipp Ternes
2025-07-28 00:58
ZenoTOF 7600 - MRM HR wiff file import parameter
ssy8827
2025-07-27 20:53
ssy8827
2025-07-27 20:53
Request improved Precision on Retention Time Prediction Values
Will Thompson
2023-02-14 13:19
Nick Shulman
2025-07-25 15:02
Missing peptide search results after importing into Skyline
Zimeng
2025-07-23 03:31
Zimeng
2025-07-24 22:32
Importing timstof PASEF small molecule data results in an instant Skyline crash
Chris Ashwood
2025-07-24 05:32
Chris Ashwood
2025-07-24 14:07
Targeted Lipidomics Analysis Help
jnl21
2025-07-24 10:21
Nick Shulman
2025-07-24 13:18
Parent mass on Peak area plot
moises zapatamr
2025-07-24 11:57
Nick Shulman
2025-07-24 12:27
Why does importing skyline from mzml /4D format fail?
z15839328872
2025-07-24 01:11
Nick Shulman
2025-07-24 07:09
Missing Tutorial Documents
akendra1
2025-07-23 11:16
Brian Connolly
2025-07-24 05:36
Why does importing skyline from mzml /4D format fail?
z15839328872
2025-07-23 23:41
Nick Shulman
2025-07-23 23:53
Why does importing skyline from mzml format fail? How to solve it
z15839328872
2025-07-22 19:54
z15839328872
2025-07-23 01:46
Clustered Report values doesn't match with manual calculation
Dhaval Patel
2025-07-22 15:12
Nick Shulman
2025-07-22 22:55
Live Report > Group Comparison
Dhaval Patel
2025-07-15 09:58
Dhaval Patel
2025-07-22 15:01
Limit on PTM losses
Juan C. Rojas E.
2025-07-22 04:58
Nick Shulman
2025-07-22 07:59
Feature requests regarding the handling of large amounts of replicates
simow02
2025-07-22 06:15
simow02
2025-07-22 06:15
SSL format for cross-links
julian bender
2025-07-16 05:45
julian bender
2025-07-21 01:18
EncyclopeDIA error
sscott13
2025-04-11 09:54
Nick Shulman
2025-07-18 08:07
Small molecule PRM transition/molecule settings
jweinb9
2025-07-17 10:42
jweinb9
2025-07-17 10:42
New Skyline version issue
anhdao darcy
2025-07-17 08:38
Nick Shulman
2025-07-17 08:46
defining same retention times for Promega 6 × 5 LC-MS/MS Peptide Reference Mix
Lauri Peil
2025-07-15 21:45
Nick Shulman
2025-07-15 22:36
DIAGONAL-PASEF
cbm11
2025-07-14 03:19
Brian Pratt
2025-07-15 12:07
Do tick marks and the number label indicate number of scans across a peak?
alena meledina
2025-07-15 01:29
alena meledina
2025-07-15 06:30
Glycopeptide analysis and NIST library import
tschilp1
2025-07-14 02:03
tschilp1
2025-07-15 00:41
Live Report Terminology Query
Dhaval Patel
2025-06-27 14:10
Dhaval Patel
2025-07-14 16:52
MAC OS Version
tranjoh1
2015-11-09 12:18
moisesleoninper
2025-07-12 19:03
Picking the correct peak failing in ~50% of my injections
sophia
2025-07-07 13:04
sophia
2025-07-11 12:23
Standard curve regression line not showing
stefan broeer
2025-06-05 21:11
stefan broeer
2025-07-10 21:31
MS1 chromatogram data missing for some peptides in PRM on an orbitrap
julian matytchak
2025-07-09 01:21
julian matytchak
2025-07-09 01:21
Thermo raw data
cntangka37533
2025-07-08 09:19
cntangka37533
2025-07-08 11:31
ID retention times incorrect
jdemeter
2025-06-19 19:56
Nick Shulman
2025-07-07 20:56
Inquiry on Bottom-Up Analysis Using Skyline for Protein/Peptide Identification
wei ng2
2025-03-06 18:18
wei ng2
2025-07-03 00:18
Ion Match Tolerance MS1 Data Waters Xevo QTOF
tateh
2025-06-30 11:08
Nick Shulman
2025-06-30 12:28
Exporting Skyline Method to UNIFI for Waters Xevo QTof G3
philipp peslalz
2025-06-30 02:09
philipp peslalz
2025-06-30 02:09
Prm conductor / expert reviewer erroring
joel steele28028
2025-06-24 20:55
philip remes
2025-06-27 12:00
signal to noise ratio/ noise information
mansour bounouba
2025-06-27 01:48
Mike MacCoss
2025-06-27 08:47
Agilent GCMS Transition Settings
bri bibel
2025-06-24 08:06
bri bibel
2025-06-26 12:40
Mass error ppm in report and RSD duplicate
chemistry Jedi
2025-06-26 01:05
chemistry Jedi
2025-06-26 01:05
Early-run m/z + IM calibration (timsTOF / any Q-TOF ?)
v delcourt
2022-06-30 07:16
Brian Pratt
2025-06-25 09:56
2025 UW Course (July)
hscprotein
2025-06-23 22:40
Mark Belanger
2025-06-24 10:45
import MS1 data failed using Skyline (64-bit) 25.1.0.142 (7401c644b4)
zhijun cao
2025-06-23 11:37
zhijun cao
2025-06-24 07:26
Chromatogram Information Unavailable
olivia schwartz
2025-06-23 16:10
Nick Shulman
2025-06-23 16:40
No automatic pick children for isotopic envelope
karamj33405
2025-06-23 11:17
Nick Shulman
2025-06-23 12:13
Unable to connect Skyline to UNIFI
bkraft
2025-06-19 08:59
bkraft
2025-06-23 08:11
Having Issues Importing Demultiplexed data into Skyline after update to v. 25.1
nikkib5
2025-06-20 11:56
Nick Shulman
2025-06-20 23:50
Problem creating library using PD3.1 results.
davidl@ohsu.edu
2025-06-06 11:23
Nick Shulman
2025-06-20 12:08
Reimporting the same files into previously analyzed Skyline file (From the last year) resulted in different areas
anokhina
2025-06-09 14:04
Brendan MacLean
2025-06-20 11:12
DIANN 2.0 speclib import failure
matthew.r.russell@manchester.ac.uk
2025-06-17 06:25
Matt Chambers
2025-06-20 11:11