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no isolation list export possible for ZenoToFF

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no isolation list export possible for ZenoToFF chi nguyen  2025-06-12 10:19
 

Hello Skyline team,
I am using skyline version 25.1 trying to work with data from a ZenoToFF 7500.
I have the precursor list ready from DIA runs analyzed in Skyline. However, when I tried to export the isolation list for a MRMHR (PRM) method on ZenoToFF, skyline keeps giving me the error "there is no isolation list data to export".

I attached here the screen shot of

  • the error messages
  • the Settings/Transition Settings/Full Scan tab
  • the File/Export Isolation list

pls let me know if there is anything else you need.

many thanks
Chi
 
 
Nick Shulman responded:  2025-06-12 20:51
It appears that the instrument type "SCIEX QTOF - Analyst" only works for DIA isolation schemes with prespecified isolation windows. It does not appear to work for PRM at all.

A different way to export a list of precursor m/z values from Skyline would be to create a custom report.
You can learn more about custom reports in Skyline here:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports
-- Nick