Oops! The information I gave you yesterday was incorrect.
The way to get the spectronaut .csv file into Skyline is with the menu item:
File > Import > Assay Library
(Yesterday I said to use "File > Import > Peptide Search", but as far as I can tell there is no way to use a Spectronaut csv file with that).
If you are still having trouble getting this to work with the "File > Import > Assay Library" menu item you can send us that .csv file and we can figure out how to make it work. If that .csv file is less than 50MB you can attach it to this support request. You can upload larger files here: