Inserting 15N-labeled products into Skyline: /home/support

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Inserting 15N-labeled products into Skyline

chemistry Jedi

2025-04-07 00:58

Hello everyone,

I would like to add the following 15N-labeled product to Skyline:

L-Tryptophan-15N₂, with the molecular formula C₁₁H₁₂¹⁵N₂O₂.

Thank you very much for your assistance.

Best regards

Nick Shulman responded:	2025-04-07 02:07
In Skyline, you would write that chemical formula as: C11H12N'2O2 You can use the symbol N' to represent 15N. The following page has some more information about heavy isotopes in Skyline: https://skyline.ms/announcements/home/support/thread.view?rowId=43912 The Small Molecule Targets tutorial is also a great place to start: https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_small_molecule -- Nick

Brian Pratt responded:	2025-04-07 02:14
This is also worth looking at: https://skyline.ms/wiki/home/software/Skyline/page.view?name=adduct_descriptions In general Skyline works in terms of a neutral formula and an adduct description specifying any labels.