• Sign In
  1. Requests

Retain only human proteins

support

View Request

view list print
Retain only human proteins akhilabrai  2025-05-14 02:52
 

Hi team,

I’m currently working with an indexed FASTA file that includes proteins from multiple species, which has led to identifications from non-human sources as well. Is there a way to filter or retain only human proteins and exclude those from other species?

Thank you.
Akhila
 
 
Mike MacCoss responded:  2025-05-14 14:45

Hi Akhila,
We do this a lot in our lab. In Skyline you can do Refine > Associate Proteins, then provide the UI with your Human fasta. Then all of the peptides will be remapped to the human sequences. The peptides not-mapped will be in the Tree and Document Grid as "Unmapped Peptides". You can then selected the "Unmapped Peptides" group and type delete.

image

I hope this helps,
Mike
 
akhilabrai responded:  2025-05-15 03:19

Ok, thank you.