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Skyline freezing with glyco-N-DIA outputsarah shapley2026-01-29 09:47Nick Shulman2026-01-29 15:17
Cannot replicate group comparison statistical test in Rkittinun l2026-01-28 18:42kittinun l2026-01-29 06:09
Bruker qTOF .damyzheng2026-01-28 15:33Brian Pratt (Claude)2026-01-29 13:14
Peptide quantitationanhdao darcy2026-01-27 13:35Nick Shulman2026-01-27 15:10
Visualising PRM data, knowing the targets of interest, but without an experimental spectral librarychloe baldreki2026-01-27 08:21chloe baldreki2026-01-27 08:55
What is the minimum computer required for running this program?theetat2026-01-26 21:37Nick Shulman2026-01-27 05:33
Unchecked "Quantitative" option for the fragment ion but included the area values in an export fileleehyoungjoo2026-01-26 13:25Nick Shulman2026-01-26 13:59
Peak integration for all transitionsSChen2026-01-26 10:01Juan C. Rojas E.2026-01-29 10:32
Incorrect quantification of low-intensity MRM peaks close to background noiseioanna kyritsi2026-01-20 01:00Nick Shulman2026-01-20 05:44
transitions/GB RAM?warham2026-01-19 11:08warham2026-01-23 08:10
Ratio to Heavy is not providing values on the calibration curvemarharyta vasnietsova2026-01-19 01:32Nick Shulman2026-01-19 07:41
is there a tutorial for TurnoverRclarkjnelson672026-01-16 19:11Nick Shulman2026-01-16 20:33
Skyline doesn't import data after DIA-NN ChromCacheBuildExceptionshubham2026-01-16 12:41shubham2026-01-18 15:19
How to use "Protein priority file" from PRM conductor?Zimeng2026-01-14 02:54philip remes2026-01-14 07:50
AutoQC config file locationphains@cmri.org.au2026-01-13 19:59phains@cmri.org.au2026-01-14 12:08
MS data not supported1963102026-01-12 16:51Nick Shulman2026-01-12 17:07
Truncated peakshadi pourhadi2026-01-12 09:08Nick Shulman2026-01-14 11:35
Hardklor/bullseye parametersrj8@uw.edu2025-12-31 11:08rj8@uw.edu2026-01-01 15:00
Importing DIA-NN into skylinerobyn eisert2025-12-18 12:26robyn eisert2025-12-18 13:03
Executing Skyline GUI with a .sky file already loadedsdelacam2025-12-18 02:17Nick Shulman2025-12-21 22:35
Why my Skyline stopped working?zhaoty2025-12-17 18:20Nick Shulman2026-01-14 07:20
Post-analysis internal calibration timsTOF data not loaded correctly in Skylinea l hipgrave ederveen2025-12-17 05:29Brian Pratt2026-01-06 09:17
Surrogate Calibration Curve Slopehelmi2025-12-16 01:26Nick Shulman2025-12-16 07:44
Bug in export with Explicit RT to waters .expper larsson2025-12-12 05:04per larsson2025-12-15 00:15
Peak boundary imputation on large datasetsTomas Vaisar2025-12-11 09:36Tomas Vaisar2025-12-11 13:15
skyline-daily not adding libraries to document via library explorerpoisson k2025-12-10 06:35Brian Pratt2025-12-10 13:25
w-ionseric bonneil2025-12-08 05:11Nick Shulman2025-12-08 08:42
DIANN 2.2 results (from HPC) upload into Skyline (windows)Tomas Vaisar2025-12-05 15:19Tomas Vaisar2025-12-05 15:48
Cannot install skyline on work computer. Supportchris brown-22025-12-05 09:01chris brown-22025-12-05 09:47
Issues with Feature Detection from RAW PRM files without searching data firstnitheshperumal2025-12-04 10:29Brian Pratt2025-12-08 13:29
URLDownloadToCacheFile error when installing Skyline and Skyline-Dailyaaron-maurais2025-12-03 10:26Nick Shulman2025-12-03 11:01
timsTOF PRM cant see MS2 in skylineguo xue2025-12-02 20:50Nick Shulman2025-12-07 20:41
Visualizing Multiple Collision Energy MS/MS Profiles in Skyline from a Single tMS2 Filedilip singh402882025-12-02 12:51dilip singh402882025-12-03 06:29
Immunopeptidomics analysis on Skyline with DDA Bruker data after MHCquant2rohit 39772025-12-01 12:29Nick Shulman2025-12-23 17:02
Is a standard curve required for relative quantificationsam lord2025-11-28 02:50Mike MacCoss2025-11-28 20:08
PRM for phosphoproteomics34583903362025-11-27 01:35Nick Shulman2025-11-27 07:15
Cannot Open Files of Raw Data?sriazisekowski2025-11-26 12:09Brian Pratt2025-11-26 12:40
Feature request: Peak Imputation WindowJuan C. Rojas E.2025-11-25 02:27Juan C. Rojas E.2025-11-25 02:27
idotp value for determing LODp de blaauw2025-11-25 02:00p de blaauw2025-11-25 02:00
Exporting Scheduled method using integrated RT?per larsson2025-11-24 09:19per larsson2025-11-24 10:37
Issue with CC Extrapolation Using Heavy Peptides Onlyangela peralbo2025-11-24 06:17Nick Shulman2025-11-24 07:34
Peptides not present in Skyline document after importing FragPipe resultstanja ziesmann2025-11-20 01:18tanja ziesmann2025-11-21 07:43
Expert Review - Thermodiogobor2025-11-19 06:54philip remes2025-11-19 09:55
Isolation offset PRM Astraljohannes fuchs133812025-11-19 02:41Nick Shulman2025-11-19 09:37
import a peptide list with RTlesurant2025-11-17 06:07Brian Pratt2025-11-17 11:09
Can I change the display format for LOQ numbers?TY2025-11-16 21:18TY2025-11-17 20:56
Flat line instead of peak MRM transition for small moleculesyasmin kadyrbekova2025-11-14 03:14Nick Shulman2025-11-14 05:29
Small molecule isotopic peakSChen2025-11-12 10:54SChen2025-11-20 06:43
Error on line XX at column YYnikolaos psychogios2025-11-12 10:49Nick Shulman2025-11-12 13:24
Filter for N-glycanssarah shapley2025-11-11 13:18Juan C. Rojas E.2025-11-12 01:57
Light to heavy ratio Calibration Curveps5622025-11-11 07:46ps5622025-11-12 13:06
Transitions for cyclic dipeptidesge78faz2025-11-11 06:05ge78faz2025-11-12 00:53
Export matched RTs and intensitieslincolnh2025-11-07 10:15Nick Shulman2025-11-18 15:51
peptide containing cystineanhdao darcy2025-11-06 08:10Mike MacCoss2025-11-06 10:53
Strategy for establishing a PRM method for intact glycopeptides using Skyline?shulian132025-11-06 07:31shulian132025-11-26 00:57
show peak areas for proteins by prec/fragmentsjdemeter2025-11-05 19:33Nick Shulman2025-11-06 02:14
Mixed sample with 18 plex TMT for PRMSONGSONG2025-11-05 11:36Nick Shulman2025-11-06 02:25
Multiple peaks viewa majcher2025-11-04 02:34Juan C. Rojas E.2025-11-05 02:16
Skyline to calculate LOD/LOQ from PRM datafloriana2025-11-03 03:43Nick Shulman2025-11-03 06:33
Issue with importing Waters SONAR data to Skylinemanohar82025-10-29 23:34Brian Pratt2025-11-06 16:35
Feature request: Make the output from "Copy Data" from the Full Scan viewer more R/Python friendlymauraisa2025-10-28 13:38Brian Pratt2025-10-29 13:29
Reinclude already excluded transition.HongSang Choi2025-10-28 13:30HongSang Choi2025-10-29 07:20
Feature request: defining peptide modification isomersJuan C. Rojas E.2025-10-28 07:26Nick Shulman2025-10-28 16:15
Possibility to unlink the integration between light and heavycarolin huber2025-10-28 05:40Nick Shulman2025-10-28 16:03
ion mobility information missing in result exporthaiyan2025-10-27 06:35Nick Shulman2025-10-28 15:14
No Collision Energy as deafult for isolation list export for Bruker timsTOF (+ request for a bonus feature)Daniel Fochtman2025-10-21 07:56Daniel Fochtman2025-10-21 07:56
Spectral library generation - is it neededmvm352025-10-21 06:03Mike MacCoss2025-10-23 08:58
Error uploading PRM .raw filemsalie416582025-10-20 09:31msalie416582025-10-20 12:39
Skyline Small Molecules - Relative Abundace for library transitionsguadalupe espadas2025-10-20 06:24guadalupe espadas2025-10-21 01:01
Using average mass for intact proteinssoren2025-10-16 11:14Nick Shulman2025-10-16 11:23
Automation for peak picking for high sample numberorsolya bilkei2025-10-15 23:39Nick Shulman2025-10-16 06:38
Custom Collision Energy Equations defaulting to precursor m/z instead of interceptChris Ashwood2025-10-10 07:51Chris Ashwood2025-10-12 17:12
Skyline Small Molecules - Agilent NLS data import failingmariacristina petrella2025-10-08 07:34mariacristina petrella2025-10-28 12:40
Installation of Skyline in MacOSsismayilova222025-10-08 05:38Nick Shulman2025-10-08 14:13
Unable to import results files from waters_connect due to authentication error.Matthew Daly2025-10-03 01:37Matthew Daly2025-10-07 08:37
viewing iRT alignment bug in -dailyWill Thompson2025-09-29 13:19Will Thompson2025-09-30 09:44
Unable to import Thermo Stellar SRM .raw: “chromatogram information unavailable” errorbusra demirtas2025-09-29 08:43Nick Shulman2025-09-29 10:31
Issue with Calibration Curve in Skylineangela peralbo2025-09-29 01:01Nick Shulman2025-09-29 07:13
how to export spectronaut generated spectral library in to skylinegupta2025-09-26 05:31Nick Shulman2025-09-26 08:41
Peptide Import Cutoff Scorepdev2025-09-25 12:09Nick Shulman2025-09-25 12:39
Difference between "Normalise to Total Ion Current" and Normalise to "Total"s subramaniam2025-09-18 07:20s subramaniam2025-09-18 17:57
Application error when importing raw dataseabiscuit2025-09-17 18:20Nick Shulman2025-09-18 16:59
Number of significant figures exported via command line reportssstoychev235132025-09-16 21:52sstoychev235132025-09-16 21:52
SkylineRunner Command?lubwen2025-09-16 16:46Nick Shulman2025-09-26 12:13
Setting working dirniklas406392025-09-12 08:39Nick Shulman2025-09-12 12:41
Transitions in PRM PASEFsusmitaghosh1919962025-09-11 04:55susmitaghosh1919962025-09-16 00:19
Transitions in PRM PASEFsusmitaghosh1919962025-09-11 04:45susmitaghosh1919962025-09-11 04:45
Pick integration with relative retention timejunyiqian2025-09-10 19:52junyiqian2025-09-10 23:17
Unable to install Skylinehudda khan632025-09-09 20:07Nick Shulman2025-09-09 21:00
安装时显示磁盘空间不足，应该需要多大的空间？怎么处理？6560539512025-09-08 19:176560539512025-09-08 19:17
Surrogate standard area displayzahall2025-09-05 08:16zahall2025-10-07 10:22
Chromatogram Unavailable for TAG Features in SkylineCmd vs GUIHarrison2025-09-01 02:15Harrison2025-09-02 23:38
DIA NN Paquet lib issuehaiyan2025-08-30 13:38haiyan2025-08-30 13:38
Importing DIANN libraryanirudhkashyap5112025-08-29 10:10Nick Shulman2025-10-20 16:44
Creating Library from Stellar GPF-DIA Fragpipe analysis Failsmaithreyan kuppusamy2025-08-28 04:57Nick Shulman2025-08-28 16:13
Altis and method exportingqin fu2025-08-27 10:37Nick Shulman2025-08-27 15:07
Import transition list from MRM assayc frampas2025-08-27 04:06Nick Shulman2025-08-29 07:00
Unable to view the chromatogram on the results importedv veerappan2025-08-25 21:57Nick Shulman2025-08-25 22:45
tSIM IQ-X wavy Chromatogramgerrit hermann2025-08-25 04:53gerrit hermann2025-09-01 01:02
为什么我安装不上这个软件啊6252764082025-08-23 00:41Nick Shulman2025-08-23 08:01