Dear Skyline team,

As the title of this request suggests, the default value that is set by Skyline for Collision Energy in an isolation list (“Export – Isolation List”) for Bruker timsTOF equals zero (i.e. 0eV). I believe that this behavior is unintended, since the timsControl software used for method generation for Bruker instruments interprets this value literally, meaning that there will be no fragmentation (though 'useless' MS2 scan will be present in the data file). To use appropriate collision energy as intended by Bruker, this CE value should default to a "-" hyphen symbol (ASCII 45). I have forgotten a few times by now to change this value in a spreadsheet manually, resulting in some wasted samples…

Since I'm already writing a post here, I would like to ask for a feature for timsTOF in Skyline specifically. It would be immensely useful if the "Retention Time - Scheduling" window would show the number of precursors per TIMS ramp and the separation of targets in RT and mobility dimensions, when ion mobility separation is utilized as selected by values in Transition Settings > Ion Mobility (I have attached a screenshot of this exact feature from Bruker software). Since ion mobility separation has an impact on the number of precursors that can be isolated and measured in a prmPASEF method, this would be much informative than a “Concurrent Precursor” plot. To illustrate for example, assuming two methods with the same number of Concurrent Precursors measured in unit time, their measurement will result in more points per chromatographic peak if the mobility of precursors is dissimilar and less points per peak if mobility of these precursors is nearly the same. That’s why this would be really helpful.

Thank you for your help in advance!