timsTOF PRM cant see MS2 in skyline

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timsTOF PRM cant see MS2 in skyline guo xue  2025-12-02 20:50
 

Hello, I have run PRM with 2 peptides sequence in timsTOF, each 2ng of peptides, which should be enough. I can observe the MS1 but there is no transition information. Is there a problem with my setting? I can upload my skyline files if needed. Thank you.

 
 
Nick Shulman responded:  2025-12-02 20:55
Yes, please send us your files.

In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.
The Share Document dialog gives you the option to include the raw data files in the .zip, which would be a good thing to do.
Alternative, you could zip up your Bruker .d folders in a separate .zip file and send them to us.

You can upload your files here:
https://skyline.ms/files.url

After we see your Skyline document and your Bruker data files we will be able to tell you why Skyline is doing what it's doing.
-- Nick
 
guo xue responded:  2025-12-03 01:20
Hi Nick, I couldn't upload to the link you shared. could you please download from my google drive with below link?
https://drive.google.com/file/d/1nFDT3UdJKuiJMVuSXCO9HKF4RkV-MOyW/view?usp=sharing
thank you