Surrogate Calibration Curve Slope

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Surrogate Calibration Curve Slope helmi  2025-12-16 01:26
 

Hello!

I have dilutions of a molecule and I would like to use the slope of the molecule for other molecules of my data set too.
But Skyline calculates its own slope for every molecule (name).

I attached a photo to explain it better: I want to use the slope of Cer 34:1 (for which I made the dilutions) for Cer 32:1 too.

So I would like to be able to just Copy Paste my slope or use a slope selection tool like for the Surrogate Standards.

Best regards
Helena

 
 
Nick Shulman responded:  2025-12-16 07:44
You cannot copy paste slopes and intercepts, but Skyline does have surrogate calibration curves.
The feature is called "Surrogate External Standards" and you can read about it at the bottom of this page:
https://skyline.ms/home/software/Skyline/wiki-page.view?name=Surrogate%20Standards

This feature will work for you if you want to use the calibration curve from another molecule.

-- Nick