Hi,

I'm posting regarding an issue with Avant-Garde for DIA using Skyline and am following the tutorial handed out at the 2019 Targeted Proteomics course in Barcelona. I believe I successfully went through all the described steps, but when I in the end pull up the Results Grid, the columns related to AvG (i.e. AvG_Score, Avg_SpectralLibSim_, etc.) do not appear. These columns are checked in the Document Settings>Annotations tab so I would expect that I can select them in the Results Grid or Document Grid, but this is not the case, even when opening the 'Customize View' option.

I'd appreciate if you could point me in the right direction in case this issue has come up in the past.
Thank you,
Franziska

Hi,
I am trying to install AvG and I already having R 3.6 installed on my computer. However, AvG always wants to install R-3.5.2. Is there a way to point it to the current R installation and only install the required packages?

Thanks,

Tomas

Hi, I get the following message whe trying to Create parameters file (TOF):

Installing Latest version of the AvantGardeDIA R package

install_Latest_AvG_RPackage<-file.path(args[5],"Update_AvG_RPackage.r")
source(install_Latest_AvG_RPackage)
Warning in install.packages(Avg_RPackage_path, repos = NULL) :
'lib = "C:/Program Files/R/R-3.5.2/library"' is not writable
Error in install.packages(Avg_RPackage_path, repos = NULL) :
unable to install packages
Calls: source -> withVisible -> eval -> eval -> install.packages
Execution halted

Dear all,

with the new skyline beta 19.0 but also with the normal skyline 4.2 I am not able to install and start AvG-DIA, which is something I would really like to do. It actully worked some time after release but when I now try to reinstall it something does not work (both skyline toolbox and zip import installation)

Could be an issue that the software does not have admin rights to write the needed R packages in the right directory under C:\Program Files\R\R-3.5.2

Could you please let me know if you have a workaround, or the info which files have to go where so that I can copy the packages manually to the correct destination.

Looking forward to get a short response.

Thank you for the tool and with best regards,
tobias

Hi,
so I just encountered a problem while trying to generate the SQLite DB for one of our datasets. I included the immediate window output. It looks like the tool expects more lines in the skyline output than there are. I tried running AvG with the demo set and another of our datasets and it worked just fine. I processed the data the same as always so I don't know what is causing this error. It would be great if you could have a look at it.
System info:
Windows 10, 64-Bit
i9-7960X @ 2.80Ghz
64 GB RAM
AvG version: 0.0.1.0
Skyline version: 4.2.0

If you need any additional info or the data itself please let me know.
Cheers,
Pascal