Welcome

The Skyline Team is pleased to announce we are bringing back our live event, the Eleventh Annual Skyline User Group Meeting, which will be held in Minneapolis, MN on Sunday afternoon before ASMS. After two years online, we are thrilled at the prospect of seeing you all in-person -- so be sure to register and attend! We would like to thank the event sponsors (see below) for their generosity and interest in collaborating with the Skyline project on exciting new targeted and quantitative mass spectrometry techniques.  

--Brendan

When: Sunday, June 5, 2022
         12:00 - 1:00 pm : Lunch served
         1:00 - 2:30 pm : Presentations
         2:30 - 3:00 pm : Snacks and break-out discussions
         3:00 - 4:30 pm : Presentations

Where: Lumber Exchange 10 South 5th Street Minneapolis, MN 55402 (map)

• From the Convention Center, head north towards South 12th Street
• Turn left on South 12th Street, walk one block
• Turn right onto South Marquette Ave
• Walk seven blocks and turn left onto South 5th Street
• Lumber Exchange (10 South 5th Street) is on the right in 1.5 blocks
• Using the 5th Street entrance, take the elevator to the 3rd floor

There is also a bus option with a stop at 2nd Ave South and Convention Center. Board the #10 and ride for five stops. Get off at Nicollet Mall & South 5th Street. Walk a half a block up to 10 South 5th Street.

Confirmed Speakers

Michael J. MacCoss, Ph.D. (University of Washington): Introduction and event host

Brendan MacLean (MacCoss Lab, University of Washington): Status of the Skyline open-source software project 13 years after its inception
The Skyline project started just after ASMS 2008 as a 2-year effort to bring better SRM/MRM software tools to the NCI-CPTAC Verification Working Group that could support the variety of mass spectrometers in use in participating laboratories. Nearly 13 years later, the Skyline project is a thriving proteomics community open-source collaboration supporting 6 mass spec instrument vendors, integrated with a wide variety of external software, with thousands of users worldwide and many thousands of instances started each week. (More info...)

Nathan Basisty, Ph.D. (NIH): Accurate Calculation of Protein Half-Lives with the TurnoveR External Tool in Skyline
Loss of protein homeostasis is a hallmark of aging and age-related conditions, including neurodegeneration, sarcopenia, and type 2 diabetes. However, alterations in markers of proteostasis machinery do not necessarily reflect rates of protein turnover. Therefore, methods to measure the turnover rates of proteins directly, rather than surrogate measurements of translation and degradation machinery, are critically needed to accurately examine the stability of the proteome during aging and disease processes. (More info...)

James Dodds,   Ph.D., (North Carolina Statue University): <a=href="https:> Improving the Speed and Selectivity of Newborn Screening using Ion Mobility Spectrometry – Mass Spectrometry (IMS-MS) analyzed via Skyline.

Detection and diagnosis of congenital disorders is the principal aim of newborn screening (NBS) programs worldwide. Mass spectrometry (MS) has become the preferred primary testing method for high-throughput NBS sampling because of its speed and selectivity. However, the ever-increasing list of NBS biomarkers included in expanding panels creates unique analytical challenges for multiplexed MS assays due to isobaric/isomeric overlap and chimeric fragmentation spectra. (More info...)