Axe Xie  Yixuan (Axe) Xie, Ph.D., is a postdoctoral research associate in the laboratory of Dr. Benjamin Garcia at Washington University School of Medicine in St. Louis, where he focused on developing MS-based methods to characterize protein and RNA modifications and understand their biological roles. He obtained his Ph.D. at the University of California, Davis, under the mentorship of Dr. Carlito Lebrilla. During his Ph.D., he established bioorthogonal tools to investigate the glycan-mediated interactions on the cell surface, as well as glycoproteomic and glycomic methods to monitor the cell glycosylation state during significant biological events.

Development of data-independent acquisition (DIA-MS) methods for Glycan and RNA modification analysis

The data-dependent acquisition (DDA) methods have been utilized to characterize biomolecules (such as proteins, RNAs, and glycocalyx). However, the information about low-abundant molecules is inconsistent and underrepresented. The limitations of DDA methods provide an opportunity for a recent MS technique, data-independent acquisition (DIA), to be used. DIA allows for the detection of both high and low abundant species. We demonstrate that DIA is a viable approach for mass spectrometry-based characterizations of RNA modifications and glycans, while Skyline can be a useful tool for analyzing the data yielded from these analyses.