QuaSAR Support 

Welcome to the QuaSAR support board. If you have a question about using QuaSAR, or if you encounter a problem, you can post your questions here.

Support is provided by the creators of the software, as time allows, though we hope others will share their experience as the user community grows.

In order to post to the forum, you'll need to sign up for an account. If you already have an account but have forgotten your password, you can reset your password using the "Forgot your password?" link on the Sign-in page.

When you post a question, please include the following information:

  • Your operating system, the version of the software you are using, and any other information that may help us to answer your question
  • A detailed description of your problem or question, including instructions for re-creating any problem that you are encountering

If you are including text output from a tool, please attach files to your message, rather than pasting in long text.


Requests 
Showing: all requestss
Calculation of LOD
(1 response) carmen.gonzaleztejedo 2017-08-31
Does anyone know how QUASAR calculates the Limit of Detection? I am trying to reproduce the results on the excel sheet called "lod-lloq-raw", but I do know how to get the LOD given from the mean and sd values present in the same sheet.

Many thanks.
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Issues with the external tool quaSAR
Don Davis 2017-07-05
Hello all,

I am currently trying to perform some small molecule MRM data analysis using QuaSAR. However, I cannot make it through QuaSAR's tutorial with peptide data due to "Error: 'opts' is deprecated. Use 'theme' instead. (Defunct; last used in version 0.9.1) execution halted" which is found in the immediate window while executing QuaSAR. I've tried using all three versions of QuaSAR and still the same error. please help.

Thanks,

Don
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Cannot install QuaSAR on 64-bit version
kathrin lauter4951 2017-01-18
Dear Skyline-Team,

I am trying to install the external Tool QuaSAR, but I always get an error (attached below) telling me that the system kannot find the required file.

I am working with the latest version of Skyline (64-bit version).

QuaSAR-1_32.zip is downloaded and then tried to be installed via the external tools-add from file.

It also does not work if installed "from tool store".

Can you help me?

Thanks a lot!!!

Kathrin
 QuaSARerror.png 
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Assay development
jdawlaty 2016-01-07
Hi,
I am developing a PRM assay with 36 SI peptide standards. I need to determine the LOD, LOQ, make calibration curves for my standards. I do not have an additional internal standard. Is it possible to determine LOD, LOQ and make calibration curves using QuaSAR in Skyline for PRM data when no internal standard is present?
I have tried to follow the tutorial supplied but I think some things in Skyline have changed since the tutorial was written. The link to the QuaSAR documentation on page 8 of the tutorial is not working.
What is the purpose of the Sample Group? It seems to be the same grouping as the concentration.
After following the tutorial the PDF with the response curves that was generated did not open on my computer.
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Sum of transitions in version 1.32
laurapas 2015-07-14
I'm not seeing the sum of transitions with version 1.3.2, but it seems to be computed when using version 1.2. Will this be fixed in a later version? I haven't seen the code for version 1.2, but works with that older version. I run into an error with no resulting computed sum of transitions within the code block "if (calc.sum.tr)" in version 1.32.

I did my own temporary fix of "if (calc.sum.tr)", but notice that sums of transition is computed when the overall audit status = NA (i.e., p-value is NA and %CV = 0). Are NAs treated as "good"?
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QaSAR offline install
zhijcao 2015-04-01
Hi there,
    R has been installed in my computer. I try to offline install QaSAR, choose from file QaSAR.zip, but it still needs to access internet for R installer and package. is there any way to install QaRAR offline?
    Thank you
zhijun
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"The system cannot find the path specified" for QuaSAR Tutorial
jie qian 2014-10-14
The QuaSAR tutorial package was downloaded and extracted to either desktop or document folder or C: disk. However, all locations give the same error message as "The system cannot find the path specified" in the immediate window.

Any suggestions?
Thanks.
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Error related to R installation for Quasar
daniel_spellman 2014-10-10
Has anyone else come accross this issue? I am recieving a series of errors while trying to intall quasar related the the version of R being installed.

Warning: unable to access index for repository http://cran.us.r-project.org/bin/windows/contrib/3.0
Warning message:
package 'bitops' is not available (for R version 3.0.1)
Warning: unable to access index for repository http://cran.us.r-project.org/bin/windows/contrib/3.0
Warning message:
package 'reshape' is not available (for R version 3.0.1)
Warning: unable to access index for repository http://cran.us.r-project.org/bin/windows/contrib/3.0
Warning message:
package 'gtools' is not available (for R version 3.0.1)
Warning: unable to access index for repository http://cran.us.r-project.org/bin/windows/contrib/3.0
Warning message:
package 'ggplot2' is not available (for R version 3.0.1)
Warning: unable to access index for repository http://cran.us.r-project.org/bin/windows/contrib/3.0
Warning message:
package 'gplots' is not available (for R version 3.0.1)
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Error running tutorial
(9 responses) nancy.finkel 2014-09-08
Hi. I am trying to run the tutorial and I am getting the following error message. Could you tell me what to do to fix this?

Best,

Nancy
 Error_message_QuaSAR_tutorial.docx 
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Errors when running tutorial
(5 responses) wildsmith.kristin 2014-04-21
I am running through the Quasar tutorial with some colleagues and we are running into a couple problems with which i hope you can help. We have all just installed the Quasar tool in skyline and have encountered errors.
1.On my PC, Quasar give an error message (slide 1) that the required columns are missing, however, the results grid is complete.

2.On the second slide, all of us are missing the "standard present" box, and for my other 2 colleagues, Quasar runs, but no response curves are generated possibly due to an error with a specific peptide (see slide 3). We attempted to delete this peptide, but actually can't find it. It appears that all other plots and csv files were generated.
Do you have any suggestions for us?
 Quasar Errors.pptx 
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QuaSAR problem
(18 responses) <2166> 2014-02-25
Hi,

We want to known how to calculate the LOD when heavy peptides are spiked in a matrix containing light peptides with unknown constant concentration.

In the panel QuaSAR, we want to use the option "none" in standard (because we don't know the concentration of light peptides)but we get an error message (joined ppt file)

Could you help us to use this option "none" and how to fill the IS spike column?

Best regards.

Luc
 error QuaSAR.pptx 
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