Deal all,
I have a problem with running QuaSAR program on Skyline
I did triplicate run for response and non response about specific drug that I defined "SampleGroup" as R and NR, "Concentration" as 5 (which is 5ug of sample), and "IS Spike" as 200 (which is 200fmol/ul).

As unit of my sample and SIS peptides are different(ug and fmol/ul), Is it applicable to put values as I mentioned above??

Also, I have one more problem with running it. Below command is error on Skyline immediate window.
Any one can help??
I also attached my Skyline file.

Thank you

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
KernSmooth 2.23 loaded
Copyright M. P. Wand 1997-2009

Attaching package: 'gplots'

The following object is masked from 'package:stats':

    lowess

[1] >>> Processing part 1 of 1
[1] Initializing ...
[1] Running AuDIT ...
Error in run.AuDIT(file, output.prefix, required.columns = audit.required.columns, :
  Could not calculate any p values.
Calls: tryCatch ... eval -> tapply -> lapply -> FUN -> FUN -> run.AuDIT
Execution halted

Finished!