Assay development

Assay development jdawlaty  2016-01-07
I am developing a PRM assay with 36 SI peptide standards. I need to determine the LOD, LOQ, make calibration curves for my standards. I do not have an additional internal standard. Is it possible to determine LOD, LOQ and make calibration curves using QuaSAR in Skyline for PRM data when no internal standard is present?
I have tried to follow the tutorial supplied but I think some things in Skyline have changed since the tutorial was written. The link to the QuaSAR documentation on page 8 of the tutorial is not working.
What is the purpose of the Sample Group? It seems to be the same grouping as the concentration.
After following the tutorial the PDF with the response curves that was generated did not open on my computer.