Peptide Import Cutoff Score

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Peptide Import Cutoff Score pdev  2025-09-25 12:09
 

When trying to do the peptide import for the tutorial, the box with "cutoff score" is not showing up on my screen, so I am unable to import the peptides and continue the tutorial. I was wondering how to get that box to show up.

 
 
Nick Shulman responded:  2025-09-25 12:17
After you choose some peptide search results files, the cutoff score will appear in the "Score threshold" column in the list of chosen files.

We got rid of the cutoff score text box a few years ago and replaced it with the thing in the grid.
This is so that if you are importing multiple different types of peptide search results, you can choose a different cutoff score for each type of results.

Which tutorial are you looking at? We were supposed to have updated the screenshots and instructions in the tutorials to reflect the new user interface. It sounds like either we forgot to update this tutorial, or you are looking at an older version of the tutorial.
-- Nick
 
pdev responded:  2025-09-25 12:25
Hi Nick,
I’m looking at the DIA-20 tutorial.
 
Nick Shulman responded:  2025-09-25 12:39
It sounds like you're looking at a .pdf file for an old version of the tutorial.
All of our current tutorials are HTML pages and you can get to them from this page on the Skyline website:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorials

-- Nick