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Tanveer Batth, Ph.D., obtained a doctorate in 2016 at the Novo Nordisk Foundation Center for Protein Research under the supervision of Professor Jesper V. Olsen. With over 15 years of proteomics experience and currently a researcher at the same institute, his most recent research has focused on utilizing proteomics to study fungal enzymes and novel post-translational modifications.
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The fungal kingdom is a source of diverse set of novel enzymes capable of performing various biochemical reactions. Consequently, fungal enzymes have been crucial biocatalysts for industrial and research purposes. However, fungal post-translational modifications have not been extensively explored.
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Here, we report the identification of a fungal N-terminal histidine methyltransferase responsible for methylating the catalytically essential N-terminal histidine of oxidative enzymes. We developed a targeted proteomics assay to screen CRISPR/Cas9 based methyltransferase knockouts in the filamentous fungi Aspergillus nidulans. Furthermore, we utilized targeted proteomics to demonstrate recombinant N-terminal histidine methylation of lytic polysaccharide monooxygenases. Skyline was utilized to process all the targeted proteomics mass spectrometry data.
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