All course schedules

2022 Skyline Online

Session 1 - Introduction to Skyline

Day 1 - Monday, Feb 7, 2022
9 am - 5 pm pm (All times EST)

  • Introduction to targeted proteomics in Skyline
  • The Skyline software ecosystem
  • Targeted MS/MS (PRM) with peptides
  • Absolute quantification and calibration curves for peptides

Day 2 - Tuesday, Feb 8, 2022
9am - 5:00 pm (All times EST)

  • Grouped study data processing
  • Intro to small molecule research in Skyline
  • Small molecule targets
  • Small molecule quantification

 

Session 2 - Advanced Small Molecules Topics

Day 1 - Thursday Feb 10, 2022
9:00 am - 5:00 pm (All times EST)

  • Skyline for small molecules
  • Method transfer and quantification
  • System suitability for small molecules
  • Advanced small molecule quantification (multiplexed panels)
  • Analysis of high resolution metabolomics data

Day 2 - Friday, Feb 11, 2022
9:00 am - 5:00 pm (All times EST)

  • Ion mobility in proteomics and metabolomics (iRT)
  • Analysis of lipidomics data in Skyline
  • Isotope incorporation / flux analysis in Skyline
  • Gas chromatography-mass spectrometry analysis
  • The future of small molecule analysis in Skyline

 

Session 3 - Introduction to Skyline

Day 1 - Monday Feb 14, 2022

9am - 5:00 pm (All times EST)

  • Introduction to targeted proteomics in Skyline
  • The Skyline ecosystem
  • Targeted MS/MS (PRM)
  • Small molecule method transfer and quantification
  • Absolute quantification and calibration curves

 

Day 2 - Tuesday Feb 15, 2022

9am - 5:00 pm (All times EST)

  • Maual data analysis
  • Using indexed retention time (iRT)
  • Grouped study data processing in Skyline
  • Using Panorama and AutoQC

 

Session 4 - Advanced Proteomics Topics

Day 1 - Thursday Feb 17, 2022

9am - 5:00 pm (All times EST)

  • Introduction to data independent acquisition (DIA)
  • Analysis of DIA data in Skyline
  • DIA instrument setup
  • Generating DIA-only libraries with EncyclopeDIA
  • IMS filtering for DDA and DIA quantitation

Day 2 - Friday Feb 18, 2022 
9am - 5:00 pm (All times EST)

  • Introduction to post transitional modifiers (PTMs)
  • Carbonyl PTM walkthrough
  • Skyline tips and tricks for PTM quantification
  • Advanced process control for big data
  • Open searching and DIA