Description:

This course will address a range of targeted techniques using selected reaction monitoring (SRM) and parallel reaction monitoring (PRM) mass spectrometry approaches for targeted quantitative proteomics and other small molecule -omics. Session topics will include an introduction to setting up target lists for peptides and small molecules, basic importing and understanding targeted mass spectrometer data, absolute quantification, differential abundance testing, and a general focus on learning to use Skyline software effectively in your research. The instructor list includes researchers and the Skyline project lead, all with a wealth of combined experience in targeted and quantitative mass spectrometry, and both using and creating Skyline to meet the needs of diverse research questions.

Instructors: Brendan MacLean, Mike MacCoss, Chris Ashwood, Tina Ludwig, Lillian Heil, Juan Rojas

Schedule:

Day 1 - Monday, February 7, 2022 
9:00 am - 5:00 pm (All times EST)

• Introduction to targeted proteomics in Skyline
• The Skyline software ecosystem
• Targeted MS/MS (PRM) with peptides
• Absolute quantification and calibration curves for peptides

Day 2 - Tuesday, February 8, 2022 
9:00 am - 5:00 pm (All times EST)

• Grouped study data processing
• Intro to small molecule research in Skyline
• Small molecule targets
• Small molecule quantification