S3 - Introduction to Targeted Proteomics: SRM/MRM and PRM

2021 Skyline Online


The course schedule will address a range of targeted techniques using selected reaction monitoring (SRM) and parallel reaction monitoring (PRM) mass spectrometry approaches for targeted quantitative proteomics. Session topics will include an introduction to mass spectrometry and proteomics, system suitability, absolute quantification, differential abundance testing, and, of course, learning to use Skyline software more effectively in your research. The instructor list includes researchers from both academia and industry with a wealth of combined experience in targeted and quantitative proteomics and using Skyline to meet the needs of diverse research questions.

Instructors: Lindsay Pino, Aparna Baxi, Stoyan Stoychev, Nick Riley, Tom Lau


Day 1 - Monday April 19, 2020 

  • Introduction to targeted proteomics in Skyline
  • The Skyline software ecosystem
  • Targeted MS/MS (PRM)
  • Absolute quantification and calibration curves

Day 2 - Tuesday April 20, 2021 

  • Manual data analysis
  • Using indexed retention time (iRT)
  • Grouped study data processing in Skyline
  • Using Panorama and AutoQC