All course schedules

2021 Skyline Online

Session 1 - Introduction to Skyline for Targeting Peptides and Small Molecules: SRM/MRM and PRM


Day 1 - Monday, April 12, 2021
 
9 am - 5 pm pm (All times EDT)

  • Introduction to targeted proteomics in Skyline
  • The Skyline software ecosystem
  • Targeted MS/MS (PRM) with peptides
  • Absolute quantification and calibration curves for peptides

Day 2 - Tueday, April 13, 2021 
9am - 5:00 pm (All times EDT)

  • Grouped study data processing
  • Intro to small molecule research in Skyline
  • Small molecule targets
  • Small molecule quantification

Session 2 - Advanced Topics in Skyline for Small Molecules


Day 1 - Thursday April 15, 2020
 
9:00 am - 5:00 pm (All times EDT)

  • Skyline for small molecules
  • Method transfer and quantification
  • System suitability for small molecules
  • Advanced small molecule quantification (multiplexed panels)
  • Analysis of high resolution metabolomics data

Day 2 - Friday, April 16, 2021
9:00 am - 5:00 pm (All times EDT)

  • Ion mobility in proteomics and metabolomics (iRT)
  • Analysis of lipidomics data in Skyline
  • Isotope incorporation / flux analysis in Skyline
  • Gas chromatography-mass spectrometry analysis
  • The future of small molecule analysis in Skyline

Session 3 - Introduction to Targeted Proteomics: SRM/MRM and PRM


Day 1 - Monday April 19, 2021
9am - 5:00 pm (All times EDT)

  • Introduction to targeted proteomics in Skyline
  • The Skyline ecosystem
  • Targeted MS/MS (PRM)
  • Small molecule method transfer and quantification
  • Absolute quantification and calibration curves

Day 2 - Tuesday April 20, 2021
9am - 5:00 pm (All times EDT)

  • Maual data analysis
  • Using indexed retention time (iRT)
  • Grouped study data processing in Skyline
  • Using Panorama and AutoQC

Session 4 - Advanced Quantitative Proteomics: DIA and PTMs


Day 1 - Thursday April 22, 2021 
9am - 5:00 pm (All times EDT)

  • Introduction to data independent acquisition (DIA)
  • Analysis of DIA data in Skyline
  • DIA instrument setup
  • Generating DIA-only libraries with EncyclopeDIA
  • IMS filtering for DDA and DIA quantitation

Day 2 - Friday April 23, 2021 
9am - 5:00 pm (All times EDT)

  • Introduction to post transitional modifiers (PTMs)
  • Carbonyl PTM walkthrough
  • Skyline tips and tricks for PTM quantification
  • Advanced process control for big data
  • Open searching and DIA