All course schedules

2021 Skyline Online

Session 1 - Introduction to Skyline for Targeting Peptides and Small Molecules: SRM/MRM and PRM


Day 1 - Monday, April 12, 2021
 
9 am - 5 pm pm (All times EDT)

  • Introduction to targeted proteomics in Skyline
  • The Skyline software ecosystem
  • Targeted MS/MS (PRM) with peptides
  • Absolute quantification and calibration curves for peptides

Day 2 - Tueday, April 13, 2021 
9am - 5:00 pm (All times EDT)

  • Grouped study data processing
  • Intro to small molecule research in Skyline
  • Small molecule targets
  • Small molecule quantification

  • Session 2 - Advanced Topics in Skyline for Small Molecules


    Day 1 - Thursday April 15, 2020
     
    9:00 am - 5:00 pm (All times EDT)

    • Skyline for small molecules
    • Method transfer and quantification
    • System suitability for small molecules
    • Advanced small molecule quantification (multiplexed panels)
    • Analysis of high resolution metabolomics data

    Day 2 - Friday, April 16, 2021
    9:00 am - 5:00 pm (All times EDT)

    • Ion mobility in proteomics and metabolomics (iRT)
    • Analysis of lipidomics data in Skyline
    • Isotope incorporation / flux analysis in Skyline
    • Gas chromatography-mass spectrometry analysis
    • The future of small molecule analysis in Skyline

    Session 3 - Introduction to Targeted Proteomics: SRM/MRM and PRM


    Day 1 - Monday April 19, 2021
    9am - 5:00 pm (All times EDT)

    • Introduction to targeted proteomics in Skyline
    • The Skyline ecosystem
    • Targeted MS/MS (PRM)
    • Small molecule method transfer and quantification
    • Absolute quantification and calibration curves

    Day 2 - Tuesday April 20, 2021
    9am - 5:00 pm (All times EDT)

    • Maual data analysis
    • Using indexed retention time (iRT)
    • Grouped study data processing in Skyline
    • Using Panorama and AutoQC

    Session 4 - Advanced Quantitative Proteomics: DIA and PTMs


    Day 1 - Thursday April 22, 2021 
    9am - 5:00 pm (All times EDT)

    • Introduction to data independent acquisition (DIA)
    • Analysis of DIA data in Skyline
    • DIA instrument setup
    • Generating DIA-only libraries with EncyclopeDIA
    • IMS filtering for DDA and DIA quantitation

    Day 2 - Friday April 23, 2021 
    9am - 5:00 pm (All times EDT)

    • Introduction to post transitional modifiers (PTMs)
    • Carbonyl PTM walkthrough
    • Skyline tips and tricks for PTM quantification
    • Advanced process control for big data
    • Open searching and DIA