James Bollinger, Ph.D., is a postdoctoral research fellow for Michael J. MacCoss in Department of Genome Sciences at the University of Washington. Originally from Chicago, he received a B.A. in Psychology and a B.S. in Biochemistry from the University of Missouri. After several years of working as a research scientist in an academic laboratory, he returned to graduate studies. Under the tutelage of Michael H. Gelb, he obtained a Ph.D in organic chemistry from the University of Washington. In his current position, he focuses his efforts on method development for targeted proteomic assays.

Multiplexing Clinical Protein Targets in Dried Blood Spots

In clinical diagnostics, dried blood spot (DBS) samples have several advantages over their serum and plasma counterparts. Despite the success of DBS/tandem MS protocols in newborn screening labs and in small-molecule drug development, there are relatively few reports of their use in clinical protein diagnostics. This talk examines the systematic development of targeted proteomic assay utilizing the DBS sample matrix. Most of the included proteins already have diagnostic assays used in the clinical laboratory as individual immunoassays or enzyme activity assays. For each assay target, an analytical standard is used to derive an optimal set of peptide proxies. I will then demonstrate the value of this empirical approach by creating and validating a selected reaction monitoring (SRM) assay for the multiplexed quantitation of a set of 50 proteins within the DBS matrix using a single protein as an internal standard.