Description:

The course schedule will address a range of targeted techniques using selected reaction monitoring (SRM) and parallel reaction monitoring (PRM) mass spectrometry approaches for targeted quantitative proteomics. Session topics will include an introduction to mass spectrometry and small molecules, system suitability, absolute quantification, differential abundance testing, and, of course, learning to use Skyline software more effectively in your research. The instructor list includes researchers from both academia and industry with a wealth of combined experience in targeted and quantitative proteomics and using Skyline to meet the needs of diverse research questions.

Instructors: Chris Ashwood, Britt Lee, Michael Davern, Kat Forrest, Haley Jostes

Schedule: 

Day 1 - Wednesday, October 8, 2025 
9:00am - 5:00pm (All times EST)

• Intro to small molecule research in Skyline
• Small molecule targets
• Small molecule quantification
• Lipidomics overview

Day 2 - Thursday, October 9, 2025 
9:00am - 5:00pm (All times EST)

• Advanced methods for small molecule research in Skyline
• Small molecule method development
• System suitability for small molecules
• Advanced small molecule quantification (multiplexed panels)
• Analysis of high resolution metabolomics data

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