Description:
The course schedule will address a range of targeted techniques using selected reaction monitoring (SRM) and parallel reaction monitoring (PRM) mass spectrometry approaches for targeted quantitative proteomics. Session topics will include an introduction to mass spectrometry and proteomics, system suitability testing, absolute quantification, differential abundance testing, and, of course, learning to use Skyline software more effectively in your research. The instructor list includes researchers from both academia and industry with a wealth of combined experience in targeted and quantitative proteomics and using Skyline to meet the needs of diverse research questions.
Instructors: TBD
Schedule:
Day 1 - Tuesday, October 14, 2025
11:00 am - 7:00 pm (All times EST)
- Introduction to targeted proteomics in Skyline
- The Skyline software ecosystem
- Designing targeted MS/MS (PRM) assays
- Absolute quantification and calibration curves
Day 2 - Wednesday, October 15, 2025
11:00 am - 7:00 pm (All times EST)
- Manual data analysis
- Using indexed retention time (iRT)
- Experimental design and analysis in Skyline
- Using Panorama and AutoQC
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