Here is the collision energy optimization tutorial:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_optimize_ce
The collision energy optimization tutorial is only available in English.
The latest released version of Skyline is 24.1. We always recommend using the latest version of Skyline unless you are trying to reproduce results from older experiments.
I can see from your screenshot that you are using an older version of Skyline because Skyline 24.1 uses different images for the green, yellow and red peak quality dots that you see next to the items in the Targets tree.
Nothing has changed in a long while in the area of SRM collision energy optimization, so the collision energy optimization tutorial will work with older versions of Skyline. However, we do recommend that you upgrade to Skyline version 24.1.
By the way, I see that many of the transitions in your Targets tree have no green, yellow, or red symbol next to them which usually indicates that there was no matching chromatogram in the data file. This often happens if your data was collected across multiple injections.
When you do "File > Import > Results" in Skyline, the first window that comes up has some options which would allow you to combine multiple injections into a single Replicate. I have circled those options in the attached screenshot.
You might also find some useful information in the Targeted Method Refinement tutorial:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_method_refine
this tutorial is also available in Chinese:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_method_refine_zh
-- Nick