Dear Skyline Support,

Enjoyed the Seattle course this year! Having an issue trying to load a DIA-NN generated spectral library to analyze within Skyline. DIA-NN exported two file formats for the spectral library - a .tsv and .speclib with skyline in it. When opening the .tsv file, there's a red exclamation for the error getting score type. When opening the .speclib file in Import Peptide Search, it recognizes q-value with 0.01 score threshold, but when I click next (with iRT standard peptides > automatic and workflow > DIA), the following error is produced. (Sorry did not see another support post with this)

---------------------------
Skyline-daily
---------------------------
ERROR: unable to determine DIA-NN report filename for 'skyline.speclib': the TSV report is required to read speclib files and must be in the same directory as the speclib and share some leading characters (e.g. somedata-tsv.speclib and somedata-report.tsv)

Command-line: C:\Users\grayl\AppData\Local\Apps\2.0\2W3HK4HC.6BC\6054QJWK.WJT\skyl..tion_9286511f3362df93_0018.0001_7968dc5caf28c22c\BlibBuild -s -A -H -v warn -o -c 0.95 -i 1002_DIANN -S "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant202407220448.stdin.txt" "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant.blib"
Working directory: H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\240720_v1
---------------------------
OK More Info
---------------------------
Skyline-daily (64-bit) 24.1.1.202 (c511d22bf)

System.IO.IOException: ERROR: unable to determine DIA-NN report filename for 'skyline.speclib': the TSV report is required to read speclib files and must be in the same directory as the speclib and share some leading characters (e.g. somedata-tsv.speclib and somedata-report.tsv)

Command-line: C:\Users\grayl\AppData\Local\Apps\2.0\2W3HK4HC.6BC\6054QJWK.WJT\skyl..tion_9286511f3362df93_0018.0001_7968dc5caf28c22c\BlibBuild -s -A -H -v warn -o -c 0.95 -i 1002_DIANN -S "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant202407220448.stdin.txt" "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant.blib"
Working directory: H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\240720_v1 ---> System.IO.IOException: ERROR: unable to determine DIA-NN report filename for 'skyline.speclib': the TSV report is required to read speclib files and must be in the same directory as the speclib and share some leading characters (e.g. somedata-tsv.speclib and somedata-report.tsv)

Command-line: C:\Users\grayl\AppData\Local\Apps\2.0\2W3HK4HC.6BC\6054QJWK.WJT\skyl..tion_9286511f3362df93_0018.0001_7968dc5caf28c22c\BlibBuild -s -A -H -v warn -o -c 0.95 -i 1002_DIANN -S "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant202407220448.stdin.txt" "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant.blib"
Working directory: H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\240720_v1

Output:
Reading results from skyline.speclib.
ERROR: unable to determine DIA-NN report filename for 'skyline.speclib': the TSV report is required to read speclib files and must be in the same directory as the speclib and share some leading characters (e.g. somedata-tsv.speclib and somedata-report.tsv)
100%

 ---> System.IO.IOException: ERROR: unable to determine DIA-NN report filename for 'skyline.speclib': the TSV report is required to read speclib files and must be in the same directory as the speclib and share some leading characters (e.g. somedata-tsv.speclib and somedata-report.tsv)

Command-line: C:\Users\grayl\AppData\Local\Apps\2.0\2W3HK4HC.6BC\6054QJWK.WJT\skyl..tion_9286511f3362df93_0018.0001_7968dc5caf28c22c\BlibBuild -s -A -H -v warn -o -c 0.95 -i 1002_DIANN -S "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant202407220448.stdin.txt" "H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\Skyline_DIANN_1002\1002_DIANN.redundant.blib"
Working directory: H:\1001_1002_Workhorse_Degraders\240719_Re-Analyze_1002\DIA-NN_1002\240720_v1
   at pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) in C:\proj\pwiz\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:line 203
   --- End of inner exception stack trace ---
   --- End of inner exception stack trace ---
   at pwiz.Common.SystemUtil.ProcessRunner.ThrowExceptionWithOutput(Exception exception, String output) in C:\proj\pwiz\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:line 263
   at pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) in C:\proj\pwiz\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:line 245
   at pwiz.BiblioSpec.BlibBuild.BuildLibrary(LibraryBuildAction libraryBuildAction, IProgressMonitor progressMonitor, IProgressStatus& status, String& commandArgs, String& messageLog, String[]& ambiguous) in C:\proj\pwiz\pwiz_tools\Shared\BiblioSpec\BlibBuild.cs:line 475
   at pwiz.Skyline.Model.Lib.BiblioSpecLiteBuilder.BuildLibrary(IProgressMonitor progress) in C:\proj\pwiz\pwiz_tools\Skyline\Model\Lib\BiblioSpecLiteBuilder.cs:line 163
---------------------------