Impossible to create my library

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Impossible to create my library cindy dieryckx  2024-07-16 00:07
 

Hello,
I'm trying to download a new library but I get this error message "ERROR: no such table: MassSpectrumItems.
I don't know what to do because it usually works.
Thank you for your help

 
 
Nick Shulman responded:  2024-07-16 08:22
I think this will work if you use Skyline-daily instead of Skyline 23.1.

You can install Skyline-daily by clicking the "Skyline-daily (beta)" button on the main Skyline page:
https://skyline.ms/project/home/software/Skyline/begin.view

The error about the table "MassSpectrumItems" indicates that your results are in the ProteomeDiscoverer 3.1 format.
Skyline 23.1 does not know how to read ProteomeDiscoverer 3.1 so you have to use Skyline-daily instead.
Skyline 24.1 will be released in a few days, and that new version will be able to read ProteomeDiscoverer 3.1 files as well.

When working with ProteomeDiscoverer 3.1 .pdresult files, it is also necessary that the file with the extension ".pdResultDetails" be present in the same folder as the .pdresult file.
-- Nick
 
cindy dieryckx responded:  2024-07-23 07:07
Hello,
Thank you for your answer, I managed to install the first library.
Now, I want to add a second one but impossible, below, the error message:
Skyline-daily (64-bit) 24.1.1.202 (c511d22bf)

System.IO.IOException: ERROR: This file does not contain q-values. You can set a cut-off score of 0 in order to build a library from it, but this may cause your library to include a lot of false-positives.
ERROR: reading file 240710-EXP-Pool1-Pool4-01.pdResult

Command-line: C:\Users\cdieryckx\AppData\Local\Apps\2.0\CLXE77NX.HGZ\O99JJYRC.TAG\skyl..tion_9286511f3362df93_0018.0001_7968dc5caf28c22c\BlibBuild -s -A -H -v warn -o -i pool4_fred -S "\\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024\pool4_fred.redundant202407230357.stdin.txt" "\\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024\pool4_fred.redundant.blib"
Working directory: \\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024 ---> System.IO.IOException: ERROR: This file does not contain q-values. You can set a cut-off score of 0 in order to build a library from it, but this may cause your library to include a lot of false-positives.
ERROR: reading file 240710-EXP-Pool1-Pool4-01.pdResult

Command-line: C:\Users\cdieryckx\AppData\Local\Apps\2.0\CLXE77NX.HGZ\O99JJYRC.TAG\skyl..tion_9286511f3362df93_0018.0001_7968dc5caf28c22c\BlibBuild -s -A -H -v warn -o -i pool4_fred -S "\\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024\pool4_fred.redundant202407230357.stdin.txt" "\\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024\pool4_fred.redundant.blib"
Working directory: \\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024

Output:
Reading results from 240710-EXP-Pool1-Pool4-01.pdResult.
ERROR: This file does not contain q-values. You can set a cut-off score of 0 in order to build a library from it, but this may cause your library to include a lot of false-positives.
ERROR: reading file 240710-EXP-Pool1-Pool4-01.pdResult
100%

 ---> System.IO.IOException: ERROR: This file does not contain q-values. You can set a cut-off score of 0 in order to build a library from it, but this may cause your library to include a lot of false-positives.
ERROR: reading file 240710-EXP-Pool1-Pool4-01.pdResult

Command-line: C:\Users\cdieryckx\AppData\Local\Apps\2.0\CLXE77NX.HGZ\O99JJYRC.TAG\skyl..tion_9286511f3362df93_0018.0001_7968dc5caf28c22c\BlibBuild -s -A -H -v warn -o -i pool4_fred -S "\\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024\pool4_fred.redundant202407230357.stdin.txt" "\\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024\pool4_fred.redundant.blib"
Working directory: \\fs.ibcp.fr\m2rm\Cindy Dieryckx\PRM\PRM lustre, sn et mec fibros psf juillet2024
   à pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) dans C:\proj\pwiz\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:ligne 203
   --- Fin de la trace de la pile d'exception interne ---
   --- Fin de la trace de la pile d'exception interne ---
   à pwiz.Common.SystemUtil.ProcessRunner.ThrowExceptionWithOutput(Exception exception, String output) dans C:\proj\pwiz\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:ligne 263
   à pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) dans C:\proj\pwiz\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:ligne 245
   à pwiz.BiblioSpec.BlibBuild.BuildLibrary(LibraryBuildAction libraryBuildAction, IProgressMonitor progressMonitor, IProgressStatus& status, String& commandArgs, String& messageLog, String[]& ambiguous) dans C:\proj\pwiz\pwiz_tools\Shared\BiblioSpec\BlibBuild.cs:ligne 475
   à pwiz.Skyline.Model.Lib.BiblioSpecLiteBuilder.BuildLibrary(IProgressMonitor progress) dans C:\proj\pwiz\pwiz_tools\Skyline\Model\Lib\BiblioSpecLiteBuilder.cs:ligne 163
Kind regards,
Cindy