Inquiries about MRM Data export

Inquiries about MRM Data export mdsir  2024-05-08 18:10

Peak intensity (height) appears in the document grid as a different value from the actual obtained data. I would like to know the reason for this.

Nick Shulman responded:  2024-05-08 18:14
Your chromatogram seems to dip below zero, which often means that you are looking at the Savitzky-Golay smoothed chromatogram.
In order to see the chromatogram that Skyline was looking at when it figured out the height you should choose "View > Transform > Interpolated".

Here's a page which talks about how Skyline calculates background and height:
-- Nick