Unable to use MSFragger for spectral library | s jager | 2023-07-31 06:52 | |||||||||||||||||||||||||||||||||
Hello, I have been using MSFragger to analyze my timstof data, and i have been trying to upload the ‘interact.pep.xml’ files as spectral library. I put the '_uncalibrated.mzML' in the parent folder as had been described previously. However, i get the following error message: "interact.pep.xml: To import an MSFragger search of timsTOF data (with ion_mobility attribute), the corresponding *_uncalibrated.mgf or *_calibrated.mgf file is required" As of version 3.6, MSFragger does not output these files, and does the mxML counterparts instead (which I have). Can I somehow solve this and import the spectral library. Or is there a workaround, for example a way i can import a list of peptide sequences with modifications, and that skyline processes this similarly to when I upload the FASTA? thanks in advance! |
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