Save error after DIA generation of assay list

support
Save error after DIA generation of assay list dkueltz  2023-04-30 20:28
 
Hi,

I am generating a new DIA assay list by opening Skyline and choosing the import dia peptide search option, the using an existing blib. The process works fine and the final assay list looks great with all spectra showing properly, etc.

However when I try to save the Skyline file I get the following error:

System.Reflection.TargetInvocationException ---> pwiz.Skyline.Util.AssumptionException
   at pwiz.Skyline.Util.Assume.Fail(String error) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Util\Util.cs:line 2080
   at pwiz.Skyline.Model.Serialization.DocumentWriter.WritePeptideGroupXml(XmlWriter writer, PeptideGroupDocNode node) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\Serialization\DocumentWriter.cs:line 154
   at pwiz.Skyline.Model.Serialization.DocumentWriter.WriteXml(XmlWriter writer) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\Serialization\DocumentWriter.cs:line 71
   at pwiz.Skyline.Model.SrmDocument.Serialize(XmlTextWriter writer, String displayName, SkylineVersion skylineVersion, IProgressMonitor progressMonitor) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\SrmDocument.cs:line 2263
   at pwiz.Skyline.Model.SrmDocument.SerializeToFile(String tempName, String displayName, SkylineVersion skylineVersion, IProgressMonitor progressMonitor) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\SrmDocument.cs:line 2238
   at pwiz.Skyline.SkylineWindow.<>c__DisplayClass753_1.<SaveDocument>b__0(IProgressMonitor progressMonitor) in C:\proj\skyline_22_2\pwiz_tools\Skyline\SkylineFiles.cs:line 1011
   at pwiz.Skyline.Controls.LongWaitDlg.RunWork(Action`1 performWork) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 254
   --- End of inner exception stack trace ---
   at pwiz.Skyline.Util.Helpers.WrapAndThrowException(Exception x) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Util\Util.cs:line 1965
   at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 202
   at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 140
   at pwiz.Skyline.SkylineWindow.SaveDocument(String fileName, Boolean includingCacheFile) in C:\proj\skyline_22_2\pwiz_tools\Skyline\SkylineFiles.cs:line 1018

Is this a bug or what could be going wrong (I checked and there is no special character in the pathname and no matter what file name and path I cannot save this Skyline file)?

Thanks for any suggestions,
Dietmar
 
 
Nick Shulman responded:  2023-04-30 21:57
Unfortunately, once you get in that state, there is no way to save your document.
There is a little bit more information here:
https://skyline.ms/announcements/home/support/thread.view?rowId=60538

This bug has something to do with some but not all of the peptides in the FASTA file having well-formed accession numbers.
If you send us your FASTA file, we might be able to tell you which parts of it are causing problems for Skyline 22.2, or it might just be easier for you to use Skyline-daily instead of Skyline 22.2.
-- Nick
 
dkueltz responded:  2023-05-01 17:43
Hi Nick,

Thanks for the reply. I tried Skyline Daily latest version and it works without problem.

Best,
Dietmar