Can you send us that .mzid file?
If that file is less than 50MB you can attach it to this support request. You can upload larger files here:
https://skyline.ms/files.url
-- Nick

Hi Nick,

I just uploaded the file to the File Sharing Folder. The file name is: Heavy_mix_45min_6.mzid.

Thanks,
Chen

Thank you for sending that file.
Could you also send us the file "Heavy_mix_45min_6.mgf"?
It's possible that you do not have that file and maybe you have a file "Heavy_mix_45min_6.mzML" or "Heavy_mix_45min_6.mzXML", in which case send us whatever file you have. But, most likely, the file we need to reproduce this error is "Heavy_mix_45min_6.mgf".
Thanks,
-- Nick

Hi Nick,

I just uploaded the mgf file.

Thanks,
Chen

I cannot get any error like that to happen.
I am attaching the .blib file which I successfully built using Skyline 22.2.

I do not have a good theory about why you might be getting the error:
Invalid cvParam accession "200000854"

Which version of Skyline are you using? Are you doing "File > Import > Peptide Search" or are you doing "Settings > Peptide Settings > Library > Build"?

I might be able to figure out what is going wrong if you send me your Skyline document.
In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files.

Maybe if you send us all of the other files which are in the same folder as "Heavy_mix_45min_6.mgf"" we might be able to figure out what is going on.

Sorry I have do not yet have any idea what might be causing the error for you.
-- Nick

Hi Nick,

Can you advise where to check the version of Skyline? I downloaded it last year, not sure which version I downloaded at that time. For spectrum library, I was using "Settings > Peptide Settings > Library > Build".

Maybe it is not Skyline issue, but other vendor issue. I tried another Byonics to generate the mzid file, Skyline was able to recognize that one. Thank you for taking a deep look at the file. If you think it still could be Skyline issue, I can send over the files per your request.

Thanks,
Chen

You can check which version of Skyline you have with the menu item:
Help > About
The current version of Skyline is 22.2.0.351
The current version of Skyline-daily is 22.2.1.425

You can install Skyline or Skyline-daily by clicking the appropriate button on this page:
https://skyline.ms/project/home/software/Skyline/begin.view
-- Nick

I see. The version I am using is: 21.2.0.425. I can upgrade it.

Thanks,
Chen

Thanks for the info. Yes, I do see that that error if I use Skyline 21.2.

The older version of BiblioSpec was choking on the "UNIMOD:854" that was in your .mzid file.

This error was first reported in the following support request last summer and was fixed around that time:
https://skyline.ms/announcements/home/support/thread.view?rowId=56252
-- Nick