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PRM-PASEF method creation, 'All targets must have an ion mobility value'

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PRM-PASEF method creation, 'All targets must have an ion mobility value' robert parker  2023-03-01
 

When creating a method for bruker timsTOF for PRM PASEF I get this message see attached.

The spectral library viewer shows the ionmobility values and I have it selected in transition settings, 'use spectral library ion mobility value when present'..

Is there some where else I need to specify this in skyline so it can creat the method?

bw and many thanks!

rob
 
 
Brian Pratt responded:  2023-03-02

Could you perhaps share the Skyline document (use File>Share>Complete to create a .sky.zip file)? That's the quickest route to an answer.

Thanks for using the Skyline support board,

Brian Pratt