|Skyline integrates low signal peak but exports a zero value||halwaseem||2023-02-02|
I am using Skyline Daily v 184.108.40.2061 to integrate small molecules using a transition list. I noticed for a few metabolites, that I manually integrated, the exported Skyline report shows zero values even though there is a peak that has been integrated. In all cases these are low signal peaks. However, within the same dataset, Skyline allows integration of noise from blanks and samples that have even lower than the biological replicates that was "zeroed out".
In the retention time comparison graph, there is a retention time window showing the sample that has a zero value. The peak area graph doesn't have a line/bar for that sample because Skyline is recording it as a zero. Please refer to attached document.
Thank you for your assistance. Let me know if there are any other details you'd like from me.