Max Height

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Max Height minyg98  2021-07-15
 

Hi, Skyline team

Is the value of max height of a peptide coming from the highest point in the borderline I set?
I thought the max height in the picture attached was around 1.2e5, but when I checked in the csv file, it was 4.3e4

-Minyeong

 
 
Nick Shulman responded:  2021-07-15
Here is a web page that describes how Skyline calculates peak height and background:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tip_peak_calc

The "background" is the lowest intensity of the chromatogram at either the start or end of the peak. The "height" value that Skyline reports is the raw height minus the background.
The reason that the height that you are seeing is so much less than you expect is that the intensity at the start and end of that peak is very high.

-- Nick
 
minyg98 responded:  2021-07-15
Nick, thanks for the help!

In your powerpoint "Triggered acquisition support", it says background subtraction does not happen if Triggered Acquisition is turned on.

Is this applied only when calculating area?

-Minyeong