|Lipid Peak Integration Boundaries||dpeake||2021-02-17|
I’m investigating using Skyline for integrating lipid annotation and QA/QC.
I have several questions regarding adjusting integration parameters, in particular, the integration window that is displayed. When integrating a batch of lipids, some windows for the same species are displayed in a wider window than others. This window appears to change when the program sets different peak boundaries. This results in missed areas or integration of the another isomer, the largest peak in the window [see attached].
I would prefer to set a single integration window and peak boundary for all samples for a given lipid, thereby reducing the need for manual intervention.