Spectral library export

Spectral library export Juergen  2020-09-02

Dear Skyline team,

recently I used Skyline to build a spectral library from mascot search results (.dat files). However, I want to use the very same library also in spectraST later. Is there any way to convert the blib format to splib? I remember, that some years (~10) ago it was possible to import these libraraies into spectraST directly. But it seems that this support was stopped in the meantime. If a direct conversion is not possible, perhaps there is a way to convert the library to an intermediate format that you are aware of?

Best regards,

Brian Pratt responded:  2020-09-02

Hi Juergen,

BLIB files are implemented as SQLite databases, so it should be possible to create a converter. You should probably consult with the SpectraST developers.

Thanks for using the Skyline support board.

Brian Pratt

Brendan MacLean responded:  2020-09-02

Others have used Blib2Ms2.exe to convert the .blib to MS2 files that can be built into a SpectraST library.

Have a look at this page for how to get a full set of BiblioSpec EXE files:


Juergen responded:  2020-09-03

Dear Brian and Brendan,

Thank you both for your suggestions. I will try with the conversion to MS2 first and consult the spectraST developers if that does not work.