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Spectral library export Juergen  2020-09-02 03:43
 

Dear Skyline team,

recently I used Skyline to build a spectral library from mascot search results (.dat files). However, I want to use the very same library also in spectraST later. Is there any way to convert the blib format to splib? I remember, that some years (~10) ago it was possible to import these libraraies into spectraST directly. But it seems that this support was stopped in the meantime. If a direct conversion is not possible, perhaps there is a way to convert the library to an intermediate format that you are aware of?

Best regards,
Juergen
 
 
Brian Pratt responded:  2020-09-02 10:10

Hi Juergen,

BLIB files are implemented as SQLite databases, so it should be possible to create a converter. You should probably consult with the SpectraST developers.

Thanks for using the Skyline support board.

Brian Pratt
 
Brendan MacLean responded:  2020-09-02 17:12

Others have used Blib2Ms2.exe to convert the .blib to MS2 files that can be built into a SpectraST library.

Have a look at this page for how to get a full set of BiblioSpec EXE files:

https://skyline.ms/project/home/software/BiblioSpec/begin.view
 
Juergen responded:  2020-09-03 00:49

Dear Brian and Brendan,

Thank you both for your suggestions. I will try with the conversion to MS2 first and consult the spectraST developers if that does not work.

Best,
Juergen