|Isotope labels on some residue||Vincent Lacasse||2020-08-31|
Hello Skyline team,
I'm currently preparing an MRM assay using an internal calibration curve with two internal standards spiked-in a different concentrations. Although I was able to add my different labels to my skyline file, some of the residue of the same amino acid are labelled and not others. For instance:
How can I tell Skyline to label only some of the amino acids? I've also tried to put it as a variable modification on the protein, however it does the same thing, it labels both amino acids.
Thank you and have a nice day!