Isotope labels on some residue | Vincent Lacasse | 2020-08-31 09:59 | |||||||||||||||
Hello Skyline team, I'm currently preparing an MRM assay using an internal calibration curve with two internal standards spiked-in a different concentrations. Although I was able to add my different labels to my skyline file, some of the residue of the same amino acid are labelled and not others. For instance: SQPGQDCR How can I tell Skyline to label only some of the amino acids? I've also tried to put it as a variable modification on the protein, however it does the same thing, it labels both amino acids. Thank you and have a nice day! |
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